ArticleBiomolecular detection and quantification2017
The continuing problem of poor transparency of reporting and use of inappropriate methods for RT-qPCR.
Article in Biomolecular detection and quantification, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
7 citing papers in PubMed.
- MIQE 2.0 and the Urgent Need to Rethink qPCR Standards.International journal of molecular sciences · 2025Article
- RT-qPCR Diagnostics: The "Drosten" SARS-CoV-2 Assay Paradigm.International journal of molecular sciences · 2021Article
- Validation of Reference Genes for Quantitative PCR in Johnsongrass (Plants (Basel, Switzerland) · 2021Article
- Article
- COVID-19 and Diagnostic Testing for SARS-CoV-2 by RT-qPCR-Facts and Fallacies.International journal of molecular sciences · 2021Article
- Acetyl-11-keto-β-boswellic acid attenuates titanium particle-induced osteogenic inhibition via activation of the GSK-3β/β-catenin signaling pathway.Theranostics · 2019Article
- A PCR-based quantitative assay for the evaluation of mRNA integrity in rat samples.Biomolecular detection and quantification · 2018Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
1 author.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Attendance at this year's European Calcified Tissue Society's (ECTS) Congress reveals that the methods used to obtain qPCR results continue to be significantly flawed and that and their reporting remain inadequate.
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Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.