Evidence map›Paper›PMID 28702010›Full record

ArticleFrontiers in microbiology2017

Comparison among the Quantification of Bacterial Pathogens by qPCR, dPCR, and Cultural Methods.

Matteo Ricchi, Cristina Bertasio, Maria B Boniotti, Nadia Vicari, Simone Russo, Michela Tilola, Marco A Bellotti, Barbara Bertasi

Abstract read
In one paragraph

Article in Frontiers in microbiology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 48 papers.

0numbers the graph read from it
0cells of the map it votes in
48citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

48 citing papers in PubMed.

  1. Article
  2. Article
  3. Article
  4. Article
  5. Article
  6. Article
  7. Article
  8. Article
  9. Article
  10. Review
  11. Quantification methods ofMicrobial cell (Graz, Austria) · 2024
    Article
  12. Designing and validation of specific primers for the quantitative detection of bacteria in sugarcane inoculant.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2023
    Article
  13. Article
  14. Article
  15. A novelFrontiers in cellular and infection microbiology · 2023
    Article
  16. Review
  17. Article
  18. Review
  19. Article
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Matteo RicchiIstituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia Romagna "Bruno Ubertini," National Reference Centre for ParatuberculosisPodenzano, Italy.
Cristina BertasioIstituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia Romagna "Bruno Ubertini," National Reference Centre for Tuberculosis from M. bovisBrescia, Italy.
Maria B BoniottiIstituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia Romagna "Bruno Ubertini," National Reference Centre for Tuberculosis from M. bovisBrescia, Italy.
Nadia VicariIstituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia Romagna, National Reference Laboratory for Tularemia, Istituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia Romagna "Bruno Ubertini"Pavia, Italy.
Simone RussoIstituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia Romagna "Bruno Ubertini," National Reference Centre for ParatuberculosisPodenzano, Italy.
Michela TilolaIstituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia Romagna "Bruno Ubertini," Reparto Tecnologie Acidi Nucleici Applicate Agli AlimentiBrescia, Italy.
Marco A BellottiIstituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia Romagna, National Reference Laboratory for Tularemia, Istituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia Romagna "Bruno Ubertini"Pavia, Italy.
Barbara BertasiIstituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia Romagna "Bruno Ubertini," Reparto Tecnologie Acidi Nucleici Applicate Agli AlimentiBrescia, Italy.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The demand for rapid methods for the quantification of pathogens is increasing. Among these methods, those based on nucleic acids amplification (quantitative PCRs) are the most widespread worldwide. Together with the qPCR, a new approach named digital PCR (dPCR), has rapidly gained importance. The aim of our study was to compare the results obtained using two different dPCR systems and one qPCR in the quantification of three different bacterial pathogens:

Indexed as

bacteriadPCRpathogensqPCRquantification

Identifiers

PMID28702010
PMCPMC5487435

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.