ArticleScientific reports2017
Free-of-Acrylamide SDS-based Tissue Clearing (FASTClear) for three dimensional visualization of myocardial tissue.
Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed, 45 citations in OpenAlex.
- aDISCO: A clearing method to enable 3D microscopy of large archival paraffin-embedded human tissue blocks.Science advances · 2026Article
- Immersion-Based Clearing and Autofluorescence Quenching in Myocardial Tissue.Microcirculation (New York, N.Y. : 1994) · 2025Article
- A multiwell plate approach to increase the sample throughput during tissue clearing.Nature protocols · 2025Review
- Distinct Capabilities in NAD Metabolism Mediate Resistance to NAMPT Inhibition in Glioblastoma.Cancers · 2024Article
- MyoLoop: Design, development and validation of a standalone bioreactor for pathophysiological electromechanical in vitro cardiac studies.Experimental physiology · 2024Article
- Cardiomyocyte orientation recovery at micrometer scale reveals long-axis fiber continuum in heart walls.The EMBO journal · 2023Article
- Visualization of Organ-Specific Lymphatic Growth: An Efficient Approach to Labeling Molecular Markers in Cleared Tissues.International journal of molecular sciences · 2023Article
- Graded mesoderm assembly governs cell fate and morphogenesis of the early mammalian heart.Cell · 2023Article
- Review
- MAX: a simple, affordable, and rapid tissue clearing reagent for 3D imaging of wide variety of biological specimens.Scientific reports · 2022Article
- Scalable tissue labeling and clearing of intact human organs.Nature protocols · 2022Review
- Analysis of age-related left ventricular collagen remodeling in living donors: Implications in arrhythmogenesis.iScience · 2022Article
- A simple optical tissue clearing pipeline for 3D vasculature imaging of the mediastinal organs in mice.International journal of experimental pathology · 2021Article
- Refined CLARITY-Based Tissue Clearing for Three-Dimensional Fibroblast Organization in Healthy and Injured Mouse Hearts.Journal of visualized experiments : JoVE · 2021Article
- Review
- Three-Dimensional Imaging in Stem Cell-Based Researches.Frontiers in veterinary science · 2021Review
- Rapid immunostaining method for three-dimensional volume imaging of biological tissues by magnetic force-induced focusing of the electric field.Brain structure & function · 2021Article
- An advanced optical clearing protocol allows label-free detection of tissue necrosisTheranostics · 2021Article
- A practical guide for investigating cardiac physiology using living myocardial slices.Basic research in cardiology · 2020Review
- Biomedical Applications of Tissue Clearing and Three-Dimensional Imaging in Health and Disease.iScience · 2020Review
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
Several pathologic conditions of the heart lead to cardiac structural remodelling. Given the high density and the opaque nature of the myocardium, deep three dimensional (3D) imaging is difficult to achieve and structural analysis of pathological myocardial structure is often limited to two dimensional images and of thin myocardial sections. Efficient methods to obtain optical clearing of the tissue for 3D visualisation are therefore needed. Here we describe a rapid, simple and versatile Free-of-Acrylamide SDS-based Tissue Clearing (FASTClear) protocol specifically designed for cardiac tissue. With this method 3D information regarding collagen content, collagen localization and distribution could be easily obtained across a whole 300 µm-thick myocardial slice. FASTClear does not induce structural or microstructural distortion and it can be combined with immunostaining to identify the micro- and macrovascular networks. In summary, we have obtained decolorized myocardial tissue suitable for high resolution 3D imaging, with implications for the study of complex cardiac tissue structure and its changes during pathology.
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