ArticleScientific reports2017
Use of Single-Frequency Impedance Spectroscopy to Characterize the Growth Dynamics of Biofilm Formation in Pseudomonas aeruginosa.
Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed.
- Diagnostic and therapeutic strategies in combating implanted medical device-associated bacterial biofilm infections.Folia microbiologica · 2025Review
- Effects of Anti-Pseudomonal Agents, Individually and in Combination, With or Without Clarithromycin, on Growth and Biofilm Formation by Antibiotic-Susceptible and -Resistant Strains ofAntibiotics (Basel, Switzerland) · 2025Article
- On the effectiveness of electrical characterization of mature Staphylococcal Biofilm.Scientific reports · 2025Article
- The effect of cCMP and cUMP on growth ofFrontiers in microbiology · 2025Article
- Quantification of Pseudomonas aeruginosa biofilms using electrochemical methods.Access microbiology · 2025Article
- Effects of Sucrose and Farnesol on Biofilm Formation byMicroorganisms · 2024Article
- Monitoring ofCurrent medical mycology · 2024Article
- Real-Time Monitoring of Biofilm Formation Using a Noninvasive Impedance-Based Method.Sensors and actuators. B, Chemical · 2023Article
- Article
- How to study biofilms: technological advancements in clinical biofilm research.Frontiers in cellular and infection microbiology · 2023Review
- A new BiofilmChip device for testing biofilm formation and antibiotic susceptibility.NPJ biofilms and microbiomes · 2021Article
- Pathogens electrogenicity as a tool for in-situ metabolic activity monitoring and drug assessment in biofilms.iScience · 2021Review
- Sensing the unreachable: challenges and opportunities in biofilm detection.Current opinion in biotechnology · 2020Review
- Electrochemical Biosensors for Determination of Colorectal Tumor Biomarkers.Micromachines · 2020Review
- The Application of Impedance Spectroscopy forViruses · 2020Article
- Lactobacilli spp.: real-time evaluation of biofilm growth.BMC microbiology · 2020Article
- Hemostasis-On-a-Chip: Impedance Spectroscopy Meets Microfluidics for Hemostasis Evaluation.Micromachines · 2019Article
- Development of anJournal of oral microbiology · 2019Article
- Bioimpedimetric analysis in conjunction with growth dynamics to differentiate aggressiveness of cancer cells.Scientific reports · 2018Article
Corrections and comments
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Impedance spectroscopy has been applied in prokaryotic and eukaryotic cytometry as a label-free method for the investigation of adherent cells. In this paper, its use for characterizing the growth dynamics of P. aeruginosa biofilms is described and compared to crystal violet staining and confocal microscopy. The method allows monitoring the growth of biofilm-forming P. aeruginosa in a continuous and label-free manner over a period of 72 h in a 96 well plate format. Impedance curves obtained for P. aeruginosa PA14 wild type and mutant strains with a transposon insertion in pqsA and pelA genes exhibited distinct phases. We propose that the slope of the declining curve following a maximum at ca. 35-40 h is a measure of biofilm formation. Transplant experiments with P. aeruginosa biofilms and paraffin suggest that the impedance also reflects pellicle formation at the liquid-air interface, a barely considered contributor to impedance. Finally, the impairment of biofilm formation upon treatment of cultures with L-arginine and with ciprofloxacin, tobramycin and meropenem was studied by single frequency impedance spectroscopy. We suggest that these findings qualify impedance spectroscopy as an additional technique to characterize biofilm formation and its modulation by small molecule drugs.
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