Trial reportMicrobiome2017
Normal milk microbiome is reestablished following experimental infection with Escherichia coli independent of intramammary antibiotic treatment with a third-generation cephalosporin in bovines.
Trial report in Microbiome, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
34 citing papers in PubMed, 94 citations in OpenAlex.
- Longitudinal analysis reveals transitions in pathogen profiles associated with mastitis in dairy cows.Veterinary research · 2025Article
- The Microbial Composition of Bovine Colostrum as Influenced by Antibiotic Treatment.Antibiotics (Basel, Switzerland) · 2025Article
- Article
- Unravelling the complexity of bovine milk microbiome: insights into mastitis through enterotyping using full-length 16S-metabarcoding.Animal microbiome · 2024Article
- The occurrence ofmSystems · 2024Article
- Vertical transfer of gut microbiota from dam to neonate calf in the early of life.Scientific reports · 2024Article
- An in vitro evaluation of the effect of antimicrobial treatment on bovine mammary microbiota.Scientific reports · 2024Article
- Bovine milk microbiota: Key players, origins, and potential contributions to early-life gut development.Journal of advanced research · 2024Review
- Persistent effects of intramammary ceftiofur treatment on the gut microbiome and antibiotic resistance in dairy cattle.Animal microbiome · 2023Article
- Similarity in milk microbiota in replicates.MicrobiologyOpen · 2023Article
- Relationship between Microflora Changes and Mammary Lipid Metabolism in Dairy Cows with Mastitis.Animals : an open access journal from MDPI · 2023Article
- Microbiota members from body sites of dairy cows are largely shared within individual hosts throughout lactation but sharing is limited in the herd.Animal microbiome · 2023Article
- Absence of changes in the milk microbiota during Escherichia coli endotoxin induced experimental bovine mastitis.Veterinary research · 2023Article
- Bovine Udder Health: From Standard Diagnostic Methods to New Approaches-A Practical Investigation of Various Udder Health Parameters in Combination with 16S rRNA Sequencing.Microorganisms · 2023Article
- Geraniol-a potential alternative to antibiotics for bovine mastitis treatment without disturbing the host microbial community or causing drug residues and resistance.Frontiers in cellular and infection microbiology · 2023Article
- A longitudinal census of the bacterial community in raw milk correlated with Staphylococcus aureus clinical mastitis infections in dairy cattle.Animal microbiome · 2022Article
- Altering Microbiomes with Hydroxyapatite Nanoparticles: A Metagenomic Analysis.Materials (Basel, Switzerland) · 2022Article
- Effect of Intramammary Dry Cow Antimicrobial Treatment on Fresh Cow's Milk Microbiota in California Commercial Dairies.Antibiotics (Basel, Switzerland) · 2022Article
- Longitudinal dynamics of the bovine udder microbiota.Animal microbiome · 2022Article
- DNA Extraction and Host Depletion Methods Significantly Impact and Potentially Bias Bacterial Detection in a Biological Fluid.mSystems · 2021Article
Corrections and comments
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Authors and funding
7 authors at 4 institutions in 4 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe use of antimicrobials in food animals and the emergence of antimicrobial resistance are global concerns. Ceftiofur is the only third-generation cephalosporin labeled for veterinary use in the USA, and it is the drug of choice in the majority of dairy farms for the treatment of mastitis. Here, we use next-generation sequencing to describe longitudinal changes that occur in the milk microbiome before, during, and after infection and treatment with ceftiofur. Twelve animals were intramammary challenged with Escherichia coli in one quarter and randomly allocated to receive intramammary treatment with ceftiofur (5d) or untreated controls. Serial samples were collected from -72 to 216 h relative to challenge from the challenged quarter, an ipsilateral quarter assigned to the same treatment group, and from a third quarter that did not undergo intervention.
resultsInfection with E. coli dramatically impacted microbial diversity. Ceftiofur significantly decreased LogCFUs but had no significant effect on the milk microbiome, rate of pathogen clearance, or somatic cell count. At the end of the study, the microbial profile of infected quarters was indistinguishable from pre-challenge samples in both treated and untreated animals. Intramammary infusion with ceftiofur did not alter the healthy milk (i.e., milk devoid of clots or serous appearance and collected from a mammary gland that shows no clinical signs of mastitis) microbiome.
conclusionsOur results indicate that the mammary gland harbors a resilient microbiome, capable of reestablishing itself after experimental infection with E. coli independent of antimicrobial treatment.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.