ArticleBioTechniques2017
Template-dependent multiple displacement amplification for profiling human circulating RNA.
Article in BioTechniques, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
18 citing papers in PubMed, 25 citations in OpenAlex.
- Full annotation of serum virome in Chinese blood donors with elevated alanine aminotransferase levels.Transfusion · 2019Trial
- Recent advances in isothermal amplification techniques for detection of animal diseases.Frontiers in veterinary science · 2026Review
- Novel Viral Sequences in a Patient with Cryptogenic Liver Cirrhosis Revealed by Serum Virome Sequencing.Viruses · 2025Article
- Direct visualization of replication and R-loop collision using single-molecule imaging.Nucleic acids research · 2024Article
- Improved single-cell genome amplification by a high-efficiency phi29 DNA polymerase.Frontiers in bioengineering and biotechnology · 2023Article
- A Fluorescent Reverse-Transcription Assay to Detect Chemical Adducts on RNA.Biochemistry · 2022Article
- Within-host quantitation of anellovirus genome complexity from clinical samples.Journal of virological methods · 2022Article
- A Review of Isothermal Amplification Methods and Food-Origin Inhibitors against Detecting Food-Borne Pathogens.Foods (Basel, Switzerland) · 2022Review
- Low-host double MDA workflow for uncultured ASFV positive blood and serum sample sequencing.Frontiers in veterinary science · 2022Article
- Isothermal Amplification of Nucleic Acids Coupled with Nanotechnology and Microfluidic Platforms for Detecting Antimicrobial Drug Resistance and Beyond.Advanced pharmaceutical bulletin · 2022Review
- Cost-Effective Transcriptome-Wide Profiling of Circular RNAs by the Improved-tdMDA-NGS Method.Frontiers in molecular biosciences · 2022Article
- Identification of Circular RNAs by Multiple Displacement Amplification and Their Involvement in Plant Development.Methods in molecular biology (Clifton, N.J.) · 2021Article
- A novel target enrichment strategy in next-generation sequencing through 7-deaza-dGTP-resistant enzymatic digestion.BMC research notes · 2020Article
- Genome-wide capture sequencing to detect hepatitis C virus at the end of antiviral therapy.BMC infectious diseases · 2020Article
- In-depth serum virome analysis in patients with acute liver failure with indeterminate etiology.Archives of virology · 2020Article
- Identification of genetic variations inThe Journal of international medical research · 2019Article
- Circular RNA Profiling by Illumina Sequencing via Template-Dependent Multiple Displacement Amplification.BioMed research international · 2019Article
- Resistance detection and re-treatment options in hepatitis C virus-related chronic liver diseases after DAA-treatment failure.Infection · 2018Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 3 institutions in 2 countries.
Funding
Abstract
Multiple displacement amplification (MDA) is widely used in whole-genome/transcriptome amplification. However, template-independent amplification (TIA) in MDA is a commonly observed phenomenon, particularly when using high concentrations of random hexamer primers and extended incubation times. Here, we demonstrate that the use of random pentamer primers with 5´ ends blocked by a C18 spacer results in MDA solely in a template-dependent manner, a technique we have named tdMDA. Together with an optimized procedure for the removal of residual genomic DNA during RNA extraction, tdMDA was used to profile circulating RNA from 0.2 mL of patient sera. In comparison to regular MDA, tdMDA demonstrated a lack of quantifiable DNA amplification in the negative control, a remarkable reduction of unmapped reads from Illumina sequencing (7 ± 10.9% versus 58.6 ± 39%, P = 0.006), and increased mapping rates of the serum transcriptome (26.9 ± 7.9% versus 5.8 ± 8.2%, P = 3.8 × 10-4). Transcriptome profiles could be used to separate patients with chronic hepatitis C virus (HCV) infection from those with HCV-associated hepatocellular carcinoma (HCC). We conclude that tdMDA should facilitate RNA-based liquid biopsy, as well as other genome studies with biological specimens having ultralow amounts of genetic material.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.