ArticleBiophysical journal2017
Quantitative Brightness Analysis of Protein Oligomerization in the Nuclear Envelope.
Article in Biophysical journal, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 26 citations in OpenAlex.
- Identification of Non-basic Matrix Domain Residues That Impact HTLV-1 Gag Membrane Targeting and Particle Release.Journal of molecular biology · 2026Article
- Statistical analysis of the autocorrelation function in fluorescence correlation spectroscopy.Biophysical journal · 2024Article
- Autocorrelation function of finite-length data in fluorescence correlation spectroscopy.Biophysical journal · 2023Article
- A molecular mechanism for LINC complex branching by structurally diverse SUN-KASH 6:6 assemblies.eLife · 2021Article
- Number and Brightness Analysis: Visualization of Protein Oligomeric State in Living Cells.Advances in experimental medicine and biology · 2021Article
- Differentiating Luminal and Membrane-Associated Nuclear Envelope Proteins.Biophysical journal · 2020Article
- Counting the Components of Protein Complexes in the Nuclear Envelope.Biophysical journal · 2020Article
- Time-shifted mean-segmented Q data of a luminal protein measured at the nuclear envelope by fluorescence fluctuation microscopy.Data in brief · 2020Article
- Identifying Heteroprotein Complexes in the Nuclear Envelope.Biophysical journal · 2020Article
- Quantitative modeling of self-oligomerization of proteins in the nuclear envelope by fluorescence fluctuation analysis.Analytical biochemistry · 2019Article
- Role of KASH domain lengths in the regulation of LINC complexes.Molecular biology of the cell · 2019Article
- Myristoylation-Dependent Palmitoylation of the Receptor Tyrosine Kinase Adaptor FRS2α.Biochemistry · 2019Article
- Protein oligomerization and mobility within the nuclear envelope evaluated by the time-shifted mean-segmented Q factor.Methods (San Diego, Calif.) · 2019Article
- Conserved SUN-KASH Interfaces Mediate LINC Complex-Dependent Nuclear Movement and Positioning.Current biology : CB · 2018Article
- Fluorescence fluctuation spectroscopy reveals differential SUN protein oligomerization in living cells.Molecular biology of the cell · 2018Article
- Molecular Insights into the Mechanisms of SUN1 Oligomerization in the Nuclear Envelope.Biophysical journal · 2018Article
- Scanning number and brightness yields absolute protein concentrations in live cells: a crucial parameter controlling functional bio-molecular interaction networks.Biophysical reviews · 2018Review
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Brightness analysis of fluorescence fluctuation experiments has been used to successfully measure the oligomeric state of proteins at the plasma membrane, in the nucleoplasm, and in the cytoplasm of living cells. Here we extend brightness analysis to the nuclear envelope (NE), a double membrane barrier separating the cytoplasm from the nucleoplasm. Results obtained by applying conventional brightness analysis to fluorescently tagged proteins within the NE exhibited an unusual concentration dependence. Similarly, the autocorrelation function of the fluorescence fluctuations exhibited unexpected changes with protein concentration. These observations motivated the application of mean-segmented Q analysis, which identified the existence of a fluctuation process distinct from molecular diffusion in the NE. We propose that small changes in the separation of the inner and outer nuclear membrane are responsible for the additional fluctuation process, as suggested by results obtained for luminal and nuclear membrane-associated EGFP-tagged proteins. Finally, we applied these insights to study the oligomerization of the luminal domains of two nuclear membrane proteins, nesprin-2 and SUN2, which interact transluminally to form a nuclear envelope-spanning linker molecular bridge known as the linker of the nucleoskeleton and cytoskeleton complex.
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