ArticlePloS one2017
Cysteine protects rabbit spermatozoa against reactive oxygen species-induced damages.
Article in PloS one, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
16 citing papers in PubMed, 50 citations in OpenAlex.
- Breed-Specific Responses of Rabbit Semen to Chilling Storage: Sperm Quality, Acrosome Status, and Oxidative Stress Biomarkers.Animals : an open access journal from MDPI · 2025Article
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- Sperm freezing damage: the role of regulated cell death.Cell death discovery · 2024Review
- Extender Supplementation with Glutathione (GSH) and Taurine ImprovesVeterinary medicine international · 2023Article
- Determination of Ram (Animals : an open access journal from MDPI · 2022Article
- Beneficial Effect of Proline Supplementation on Goat Spermatozoa Quality during Cryopreservation.Animals : an open access journal from MDPI · 2022Article
- Cysteine protected cells from HWorld journal of microbiology & biotechnology · 2022Article
- Cysteine improves boar sperm quality via glutathione biosynthesis during the liquid storage.Animal bioscience · 2022Article
- Strategies for Highly Efficient Rabbit Sperm Cryopreservation.Animals : an open access journal from MDPI · 2021Review
- Article
- Proline Protects Boar Sperm against Oxidative Stress through Proline Dehydrogenase-Mediated Metabolism and the Amine Structure of Pyrrolidine.Animals : an open access journal from MDPI · 2020Article
- Sperm Antioxidant Biomarkers and Their Correlation with Clinical Condition and Lifestyle with Regard to Male Reproductive Potential.Journal of clinical medicine · 2020Article
- Apolipoprotein-J blocks increased cell injury elicited by ox-LDL via inhibiting ROS-CaMKII pathway.Lipids in health and disease · 2019Article
- Distribution of Polysulfide in Human Biological Fluids and Their Association with Amylase and Sperm Activities.Molecules (Basel, Switzerland) · 2019Article
- Resveratrol Improves Boar Sperm Quality via 5'AMP-Activated Protein Kinase Activation during Cryopreservation.Oxidative medicine and cellular longevity · 2019Article
- Glycogen Synthase Kinase-3 Regulates Sperm Motility and Acrosome Reaction via Affecting Energy Metabolism in Goats.Frontiers in physiology · 2019Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The process of cryopreservation results in over-production of reactive oxygen species, which is extremely detrimental to spermatozoa. The aim of this study was to investigate whether addition of cysteine to freezing extender would facilitate the cryosurvival of rabbit spermatozoa, and if so, how cysteine protects spermatozoa from cryodamages. Freshly ejaculated semen was diluted with Tris-citrate-glucose extender supplemented with different concentrations of cysteine. The motility, intact acrosomes, membrane integrity, mitochondrial potentials, 8-hydroxyguanosine level and sperm-zona pellucida binding capacity were examined. Furthermore, glutathione peroxidase (GPx) activity, glutathione content (GSH), and level of reactive oxygen species (ROS) and hydrogen peroxide of spermatozoa were analyzed. The values of motility, intact acrosomes, membrane integrity, mitochondrial potentials and sperm-zona pellucida binding capacity of the frozen-thawed spermatozoa in the treatment of cysteine were significantly higher than those of the control. Addition of cysteine to extenders improved the GPx activity and GSH content of spermatozoa, while lowered the ROS, DNA oxidative alterations and lipid peroxidation level, which makes spermatozoa avoid ROS to attack DNA, the plasma membrane and mitochondria. In conclusion, cysteine protects spermatozoa against ROS-induced damages during cryopreservation and post-thaw incubation. Addition of cysteine is recommended to facilitate the improvement of semen preservation for the rabbit breeding industry.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.