ArticleEnvironmental science & technology2017
Quantitative CrAssphage PCR Assays for Human Fecal Pollution Measurement.
Article in Environmental science & technology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 142 papers, 1 of them a synthesis that pooled it.
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Who cites it
142 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Have genetic targets for faecal pollution diagnostics and source tracking revolutionized water quality analysis yet?FEMS microbiology reviews · 2023Pooled it
- Accounting for methodological variabilities in disease outbreak surveillance using wastewater-based epidemiology: a case study with SARS-CoV-2.Analytical and bioanalytical chemistry · 2026Article
- Wastewater-Based Epidemiology of Hepatitis A in Mexico: Temporal Association with Hospital Admissions and Clinical-Environmental Phylogenetic Relatedness.Food and environmental virology · 2026Article
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- Viral Contaminants in a Philippine Wastewater Treatment Plant: Quantification and Treatment Reduction.Food and environmental virology · 2026Article
- Comparative Evaluation of DNA Extraction Workflows for Efficient Recovery of pBI143 from Wastewater.Food and environmental virology · 2026Article
- Wastewater-Based Surveillance of Respiratory Viruses in the SARS-CoV-2 Post-Pandemic Period in Mexico.Viruses · 2026Article
- A Century-Old Solution for 21st Century Challenges: Current Applications with a Focus on Biocontrol, Environmental Impacts, and Regulatory Perspectives.Antibiotics (Basel, Switzerland) · 2026Review
- Comparative performance of activated sludge and waste stabilization ponds for the removal of pollutants and pathogens in full-scale wastewater treatment plants in Egypt.Scientific reports · 2026Article
- Environmental investigation of a hepatitis A outbreak associated with spring water consumption in an Anabaptist community: Kentucky, 2018-2019.Journal of water and health · 2026Article
- A universal kinetic framework for quantitative isothermal amplification governed by polymerase speed, amplicon size, and binding efficiency.Nucleic acids research · 2026Article
- KlebPhaCol: a community-driven resource for Klebsiella research identified a novel phage family.Nucleic acids research · 2025Article
- Comparative Evaluation of Crassphage, Pepper Mild Mottle Virus, and Human Mastadenovirus as Indicators of Human Fecal Contamination in Surface Water.Food and environmental virology · 2025Article
- Novel Bacteroides bacteriophage-like quantitative real-time PCR assays for human sewage pollution measurement.Environmental pollution (Barking, Essex : 1987) · 2025Article
- The prototypic crAssphage is a linear phage-plasmid.Cell host & microbe · 2025Article
- Hospital wastewater surveillance for SARS-CoV-2 identifies intra-hospital dynamics of viral transmission and evolution.Applied and environmental microbiology · 2025Article
- CrAssphage as a Human Enteric Viral Contamination Bioindicator in Marketed Bivalve Mollusks.Viruses · 2025Article
- Aircraft lavatory wastewater surveillance for movement of antimicrobial resistance genes: a proof-of-concept study.Microbiology spectrum · 2025Article
- Comparative Assessment of Wastewater-Based Surveillance Normalization Methods to Improve Pathogen Monitoring in Rural Sewersheds.Environmental science & technology · 2025Article
82 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
Environmental waters are monitored for fecal pollution to protect public health and water resources. Traditionally, general fecal-indicator bacteria are used; however, they cannot distinguish human fecal waste from other animal pollution sources. Recently, a novel bacteriophage, crAssphage, was discovered by metagenomic data mining and reported to be abundant in and closely associated with human fecal waste. To confirm bioinformatic predictions, 384 primer sets were designed along the length of the crAssphage genome. Based on initial screening, two novel crAssphage qPCR assays (CPQ_056 and CPQ_064) were designed and evaluated in reference fecal samples and water matrices. The assays exhibited high specificities (98.6%) when tested against an animal fecal reference library, and crAssphage genetic markers were highly abundant in raw sewage and sewage-impacted water samples. In addition, CPQ_056 and CPQ_064 performance was compared to HF183/BacR287 and HumM2 assays in paired experiments. Findings confirm that viral crAssphage qPCR assays perform at a similar level to well-established bacterial human-associated fecal-source-identification approaches. These new viral-based assays could become important water quality management and research tools.
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Registered trials
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