ArticleSpine2017
Quantifying Baseline Fixed Charge Density in Healthy Human Cartilage Endplate: A Two-point Electrical Conductivity Method.
Article in Spine, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
What it found
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Who cites it
15 citing papers in PubMed.
- Characterizing the Baseline Regional Biphasic Mechanical Properties of Cervical Intervertebral Discs.Annals of biomedical engineering · 2025Article
- Article
- Endplate remodeling: a key indicator of cigarette smoke exposure-induced intervertebral disc degeneration in a male rat model.JBMR plus · 2025Article
- Characterizing baseline fixed charge density in human cervical intervertebral discs.Journal of biomechanics · 2025Article
- Effect of cigarette smoke exposure and cessation on regional diffusion properties in rat intervertebral discs.JOR spine · 2024Article
- Global and local identifiability analysis of a nonlinear biphasic constitutive model in confined compression.Journal of the Royal Society, Interface · 2024Article
- Article
- Regional structure-function relationships of lumbar cartilage endplates.Journal of biomechanics · 2024Article
- Mechanical loading and hyperosmolarity as a daily resetting cue for skeletal circadian clocks.Nature communications · 2023Article
- Diffusivity of Human Cartilage Endplates in Healthy and Degenerated Intervertebral Disks.Journal of biomechanical engineering · 2023Article
- Nanowarming and ice-free cryopreservation of large sized, intact porcine articular cartilage.Communications biology · 2023Article
- Shear strain and inflammation-induced fixed charge density loss in the knee joint cartilage following ACL injury and reconstruction: A computational study.Journal of orthopaedic research : official publication of the Orthopaedic Research Society · 2022Article
- Electrically Conductive and 3D-Printable Oxidized Alginate-Gelatin Polypyrrole:PSS Hydrogels for Tissue Engineering.Advanced healthcare materials · 2021Article
- A method for measuring intra-tissue swelling pressure using a needle micro-osmometer.European cells & materials · 2020Article
- Electrical Conductivity Method to Determine Sexual Dimorphisms in Human Temporomandibular Disc Fixed Charge Density.Annals of biomedical engineering · 2018Article
Corrections and comments
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Authors and funding
8 authors.
Funding
Abstract
STUDY
designRegional measurements of fixed charge densities (FCDs) of healthy human cartilage endplate (CEP) using a two-point electrical conductivity approach.
objectiveThe aim of this study was to determine the FCDs at four different regions (central, lateral, anterior, and posterior) of human CEP, and correlate the FCDs with tissue biochemical composition. SUMMARY OF BACKGROUND DATA: The CEP, a thin layer of hyaline cartilage on the cranial and caudal surfaces of the intervertebral disc, plays an irreplaceable role in maintaining the unique physiological mechano-electrochemical environment inside the disc. FCD, arising from the carboxyl and sulfate groups of the glycosaminoglycans (GAG) in the extracellular matrix of the disc, is a key regulator of the disc ionic and osmotic environment through physicochemical and electrokinetic effects. Although FCDs in the annulus fibrosus (AF) and nucleus pulposus (NP) have been reported, quantitative baseline FCD in healthy human CEP has not been reported.
methodsCEP specimens were regionally isolated from human lumbar spines. FCD and ion diffusivity were concurrently investigated using a two-point electrical conductivity method. Biochemical assays were used to quantify regional GAG and water content.
resultsFCD in healthy human CEP was region-dependent, with FCD lowest in the lateral region (P = 0.044). Cross-region FCD was 30% to 60% smaller than FCD in NP, but similar to the AF and articular cartilage (AC). CEP FCD (average: 0.12 ± 0.03 mEq/g wet tissue) was correlated with GAG content (average: 31.24 ± 5.06 μg/mg wet tissue) (P = 0.005). In addition, the cross-region ion diffusivity in healthy CEP (2.97 ± 1.00 × 10 cm/s) was much smaller than the AF and NP.
conclusionHealthy human CEP acts as a biomechanical interface, distributing loads between the bony vertebral body and soft disc tissues and as a gateway impeding rapid solute diffusion through the disc. LEVEL OF EVIDENCE: N/A.
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