Evidence map›Paper›PMID 28699091›Full record

ArticleClinical oral investigations2018

The effects of ozone application on genotoxic damage and wound healing in bisphosphonate-applied human gingival fibroblast cells.

Sıdıka Sinem Akdeniz, E Beyler, Y Korkmaz, E Yurtcu, U Ates, K Araz, F I Sahin, O Y Torun

Abstract read
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In one paragraph

Article in Clinical oral investigations, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers, 2 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
14citing papers in PubMed, 2 pooled it
3.4field-weighted citation impact, top 8% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

14 citing papers in PubMed, 2 syntheses or guidelines pooled it, 35 citations in OpenAlex.

  1. Pooled it
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  13. Ozone Therapy for Oral Palatal Ulcer in a Leukaemic Patient.European journal of case reports in internal medicine · 2020
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 1 institution in 1 country.

Sıdıka Sinem AkdenizDepartment of Oral and Maxillofacial Surgery, Faculty of Dentistry, Baskent University, Bahçelievler 11. Sokak no:26 Mareşal Fevzi Çakmak Caddesi Çankaya, 06490, Ankara, Turkey. sdksoydan@yahoo.com.
E BeylerDepartment of Oral and Maxillofacial Surgery, Faculty of Dentistry, Baskent University, Bahçelievler 11. Sokak no:26 Mareşal Fevzi Çakmak Caddesi Çankaya, 06490, Ankara, Turkey.
Y KorkmazDepartment of Medical Biology, Faculty of Medicine, Baskent University, Ankara, Turkey.
E YurtcuDepartment of Medical Biology, Faculty of Medicine, Baskent University, Ankara, Turkey.
U AtesPrivate Practice, Ankara, Turkey.
K ArazDepartment of Oral and Maxillofacial Surgery, Faculty of Dentistry, Baskent University, Bahçelievler 11. Sokak no:26 Mareşal Fevzi Çakmak Caddesi Çankaya, 06490, Ankara, Turkey.
F I SahinDepartment of Medical Genetics, Faculty of Medicine, Baskent University, Ankara, Turkey.
O Y TorunPrivate Practice, Ankara, Turkey.
Başkent University · TR

Funding

Baskent Üniversitesi (TR) (Baskent University Institutional Review Board and Ethics Committee) Project No: D-DA 13/02
6 · The paper itself

Abstract

objectivesMedication-related osteonecrosis of the jaws (MRONJ) is an extremely therapy-resistant disease involving the jaws especially following bisphosphonate treatment. Bisphosphonates accumulate in bone in concentrations sufficient to be directly toxic to the oral epithelium. Current therapeutic options are inadequate for the prevention and treatment of MRONJ. The aim of this study was to investigate effects of ozone gas plasma therapy on wound healing in bisphosphonate-applied human fibroblasts. MATERIAL AND

methodsHuman primary gingival fibroblasts were cultured. Cytotoxic concentrations (IC50) of bisphosphonates (pamidronate (PAM), alendronate (ALN), and zoledronate (ZOL)) were determined by MTT test. A 60 μg/μl for 30 s of ozone gas plasma application was performed to all experimental culture flasks after drug treatment at 24-h intervals as 3 s/cm

resultsPAM, ALN, and ZOL applications caused genotoxic damage on primary human gingival fibroblast DNA. Ozone gas plasma therapy significantly decreased the genotoxic damage (p < 0.05), and this application provided 25, 29, and 27% less genotoxic damage in order of ALN, PAM, and ZOL groups. Ozone gas plasma therapy significantly increased wound healing rates both in postsurgical 24th and 48th hours for all doses of experimental drug groups (p < 0.05).

conclusionThe ozone gas plasma application decreased genotoxic damage effect of bisphosphonate usage while improved the wound closure rate on human gingival fibroblasts. CLINICAL RELEVANCE: Ozone gas plasma therapy may be helpful in prevention of gingival healing delay in MRONJ pathogenesis especially when applied simultaneously with surgical intervention.

Indexed as

Bisphosphonate-Associated Osteonecrosis of the JawCells, CulturedDiphosphonatesFibroblastsGingivaHumansIn Vitro TechniquesMutagenicity TestsMutagensOzonePlasma GasesWound HealingDiphosphonatesMutagensOzonePlasma GasesBisphosphonate-related osteonecrosisBisphsophonateComet assayMRONJ treatmentOzone therapyScratch assay

Identifiers

PMID28699091
OpenAlexW2735959432

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.