ArticleScientific reports2017
Barcode ITS2: a useful tool for identifying Trachelospermum jasminoides and a good monitor for medicine market.
Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed.
- Prevalence and Molecular Characterization of Ascaridoid Parasites of PhilippineJournal of nematology · 2022Article
- Specification and DNA Barcoding of Thai Traditional Remedy for Chronic Kidney Disease: Pikad Tri-phol-sa-mut-than.Plants (Basel, Switzerland) · 2021Article
- Newly Emerging Pest in China,Insects · 2021Article
- Verification of Thai ethnobotanical medicine "Kamlang Suea Khrong" driven by multiplex PCR and powerful TLC techniques.PloS one · 2021Article
- Efficacy of DNA barcode internal transcribed spacer 2 (ITS 2) in phylogenetic study ofPhysiology and molecular biology of plants : an international journal of functional plant biology · 2020Article
- DNA barcodes for delineating Clerodendrum species of North East India.Scientific reports · 2020Article
- Assessing the Identity of Commercial Herbs From a Cambodian Market Using DNA Barcoding.Frontiers in pharmacology · 2020Article
- Genetic Diversity and Identification of VietnameseBiology · 2019Article
- Machine learning approaches outperform distance- and tree-based methods for DNA barcoding of Pterocarpus wood.Planta · 2019Article
- ITS2: An Ideal DNA Barcode for the Arid Medicinal Plant Rhazya Stricta.Pharmaceutical medicine · 2019Article
- Mitigating the Impact of Admixtures in Thai Herbal Products.Frontiers in pharmacology · 2019Review
- Constructing a DNA barcode reference library for southern herbs in China: A resource for authentication of southern Chinese medicine.PloS one · 2018Article
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Authors and funding
11 authors.
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Abstract
Trachelospermum jasminoides is commonly used in traditional Chinese medicine. However, the use of the plant's local alternatives is frequent, causing potential clinical problems. The T. jasminoides sold in the medicine market is commonly dried and sliced, making traditional identification methods difficult. In this study, the ITS2 region was evaluated on 127 sequences representing T. jasminoides and its local alternatives according to PCR and sequencing rates, intra- and inter-specific divergences, secondary structure, and discrimination capacity. Results indicated the 100% success rates of PCR and sequencing and the obvious presence of a barcoding gap. Results of BLAST 1, nearest distance and neighbor-joining tree methods showed that barcode ITS2 could successfully identify all the texted samples. The secondary structures of the ITS2 region provided another dimensionality for species identification. Two-dimensional images were obtained for better and easier identification. Previous studies on DNA barcoding concentrated more on the same family, genus, or species. However, an ideal barcode should be variable enough to identify closely related species. Meanwhile, the barcodes should also be conservative in identifying distantly related species. This study highlights the application of barcode ITS2 in solving practical problems in the distantly related local alternatives of medical plants.
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