ArticleScientific reports2017
Laminin-511 and -521-based matrices for efficient ex vivo-expansion of human limbal epithelial progenitor cells.
Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
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Who cites it
38 citing papers in PubMed, 58 citations in OpenAlex.
- Surface modified electrospun scaffold supports iPSC-derived limbal stem cell function.npj biomedical innovations · 2026Article
- Limbal Epithelial Stem Cells in Review: Immune and Lymphangiogenic Privilege and Their Clinical Relevance.Cells · 2026Review
- Article
- PDGFRβ in Stem Cell Microenvironments: Cross-Tissue Evidence and Hypotheses for the Limbal Stem Cell Niche.Stem cells international · 2026Review
- Proteomic Insights into Human Limbal Epithelial Progenitor-Derived Small Extracellular Vesicles.Stem cell reviews and reports · 2025Article
- Caveolin 1 and 2 enhance the proliferative capacity of BCAM-positive corneal progenitors.Scientific reports · 2025Article
- Article
- The impact of the limbal niche interactions on the self-renewal capability of limbal epithelial stem cells.Frontiers in cell and developmental biology · 2025Article
- Article
- Squishy matters - Corneal mechanobiology in health and disease.Progress in retinal and eye research · 2024Review
- Influence of Organ Culture on the Characteristics of the Human Limbal Stem Cell Niche.International journal of molecular sciences · 2023Article
- Fabrication and Characterisation of Hydrogels with Reversible Wrinkled Surfaces for Limbal Study and Reconstruction.Gels (Basel, Switzerland) · 2023Article
- Manufacturing of human corneal endothelial grafts.The ocular surface · 2023Article
- Derivation of Limbal Stem Cells from Human Adult Mesenchymal Stem Cells for the Treatment of Limbal Stem Cell Deficiency.International journal of molecular sciences · 2023Article
- Laminin-511 Activates the Human Induced Pluripotent Stem Cell Survival via α6β1 Integrin-Fyn-RhoA-ROCK Signaling.Stem cells and development · 2022Article
- Limbal BCAM expression identifies a proliferative progenitor population capable of holoclone formation and corneal differentiation.Cell reports · 2022Article
- Hyaluronan supports the limbal stem cell phenotype during ex vivo culture.Stem cell research & therapy · 2022Article
- Laminin N-terminus α31 expression during development is lethal and causes widespread tissue-specific defects in a transgenic mouse model.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2022Article
- P-Cadherin Is Expressed by Epithelial Progenitor Cells and Melanocytes in the Human Corneal Limbus.Cells · 2022Article
- Specific decellularized extracellular matrix promotes the plasticity of human ocular surface epithelial cells.Frontiers in medicine · 2022Article
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Authors and funding
7 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Optimization of culture conditions for human limbal epithelial stem/progenitor cells (LEPC) that incorporate the in vivo cell-matrix interactions are essential to enhance LEPC ex vivo-expansion and transplantation efficiency. Here, we investigate the efficacy of laminin (LN) isoforms preferentially expressed in the limbal niche as culture matrices for epithelial tissue engineering. Analyses of expression patterns of LN chains in the human limbal niche provided evidence for enrichment of LN-α2, -α3, -α5, -β1, -β2, -β3, -γ1, -γ2 and -γ3 chains in the limbal basement membrane, with LN-α5 representing a signature component specifically produced by epithelial progenitor cells. Recombinant human LN-521 and LN-511 significantly enhanced in vitro LEPC adhesion, migration and proliferation compared to other isoforms, and maintained phenotype stability. The bioactive LN-511-E8 fragment carrying only C-terminal domains showed similar efficacy as full-length LN-511. Functional blocking of α3β1 and α6β1 integrins suppressed adhesion of LEPC to LN-511/521-coated surfaces. Cultivation of LEPC on fibrin-based hydrogels incorporating LN-511-E8 resulted in firm integrin-mediated adhesion to the scaffold and well-stratified epithelial constructs, with maintenance of a progenitor cell phenotype in their (supra)basal layers. Thus, the incorporation of chemically defined LN-511-E8 into biosynthetic scaffolds represents a promising approach for xeno-free corneal epithelial tissue engineering for ocular surface reconstruction.
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