Evidence map›Paper›PMID 28698179›Full record

ArticleCirculation research2017

A Novel Regulatory Mechanism of Smooth Muscle α-Actin Expression by NRG-1/circACTA2/miR-548f-5p Axis.

Yan Sun, Zhan Yang, Bin Zheng, Xin-Hua Zhang, Man-Li Zhang, Xue-Shan Zhao, Hong-Ye Zhao, Toru Suzuki, Jin-Kun Wen

Erratum issuedOpen access · bronzeAbstract read
PubMed Publisher
In one paragraph

Article in Circulation research, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 79 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
79citing papers in PubMed, 1 pooled it
5.4field-weighted citation impact, top 3% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

79 citing papers in PubMed, 1 synthesis or guideline pooled it, 129 citations in OpenAlex.

  1. Pooled it
  2. Increased ATCell death & disease · 2020
    Trial
  3. Review
  4. Review
  5. Review
  6. Article
  7. Article
  8. Article
  9. Article
  10. Article
  11. Article
  12. Review
  13. Selenomethionine Suppress the Progression of Poorly Differentiated Thyroid CancerCombinatorial chemistry & high throughput screening · 2024
    Article
  14. Emerging Role of Circular RNAs in Hepatocellular Carcinoma Immunotherapy.International journal of molecular sciences · 2023
    Review
  15. Article
  16. Article
  17. Article
  18. Review
  19. Review
  20. Article

19 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

9 authors at 2 institutions in 1 country.

Yan SunFrom the Department of Biochemistry and Molecular Biology, The Key Laboratory of Neural and Vascular Biology, Ministry of Education of China, Hebei Medical University (Y.S., Z.Y., B.Z., X.-h.Z., M.-l.Z., X.-s.Z., H.-y.Z., J.-k.W.); Department of Urology, The Second Hospital of Hebei Medical University, China (Z.Y.); and Department of Cardiovascular Sciences, University of Leicester, United Kingdom (T.S.).
Zhan YangFrom the Department of Biochemistry and Molecular Biology, The Key Laboratory of Neural and Vascular Biology, Ministry of Education of China, Hebei Medical University (Y.S., Z.Y., B.Z., X.-h.Z., M.-l.Z., X.-s.Z., H.-y.Z., J.-k.W.); Department of Urology, The Second Hospital of Hebei Medical University, China (Z.Y.); and Department of Cardiovascular Sciences, University of Leicester, United Kingdom (T.S.).
Bin ZhengFrom the Department of Biochemistry and Molecular Biology, The Key Laboratory of Neural and Vascular Biology, Ministry of Education of China, Hebei Medical University (Y.S., Z.Y., B.Z., X.-h.Z., M.-l.Z., X.-s.Z., H.-y.Z., J.-k.W.); Department of Urology, The Second Hospital of Hebei Medical University, China (Z.Y.); and Department of Cardiovascular Sciences, University of Leicester, United Kingdom (T.S.).
Xin-Hua ZhangFrom the Department of Biochemistry and Molecular Biology, The Key Laboratory of Neural and Vascular Biology, Ministry of Education of China, Hebei Medical University (Y.S., Z.Y., B.Z., X.-h.Z., M.-l.Z., X.-s.Z., H.-y.Z., J.-k.W.); Department of Urology, The Second Hospital of Hebei Medical University, China (Z.Y.); and Department of Cardiovascular Sciences, University of Leicester, United Kingdom (T.S.).
Man-Li ZhangFrom the Department of Biochemistry and Molecular Biology, The Key Laboratory of Neural and Vascular Biology, Ministry of Education of China, Hebei Medical University (Y.S., Z.Y., B.Z., X.-h.Z., M.-l.Z., X.-s.Z., H.-y.Z., J.-k.W.); Department of Urology, The Second Hospital of Hebei Medical University, China (Z.Y.); and Department of Cardiovascular Sciences, University of Leicester, United Kingdom (T.S.).
Xue-Shan ZhaoFrom the Department of Biochemistry and Molecular Biology, The Key Laboratory of Neural and Vascular Biology, Ministry of Education of China, Hebei Medical University (Y.S., Z.Y., B.Z., X.-h.Z., M.-l.Z., X.-s.Z., H.-y.Z., J.-k.W.); Department of Urology, The Second Hospital of Hebei Medical University, China (Z.Y.); and Department of Cardiovascular Sciences, University of Leicester, United Kingdom (T.S.).
Hong-Ye ZhaoFrom the Department of Biochemistry and Molecular Biology, The Key Laboratory of Neural and Vascular Biology, Ministry of Education of China, Hebei Medical University (Y.S., Z.Y., B.Z., X.-h.Z., M.-l.Z., X.-s.Z., H.-y.Z., J.-k.W.); Department of Urology, The Second Hospital of Hebei Medical University, China (Z.Y.); and Department of Cardiovascular Sciences, University of Leicester, United Kingdom (T.S.).
Toru SuzukiFrom the Department of Biochemistry and Molecular Biology, The Key Laboratory of Neural and Vascular Biology, Ministry of Education of China, Hebei Medical University (Y.S., Z.Y., B.Z., X.-h.Z., M.-l.Z., X.-s.Z., H.-y.Z., J.-k.W.); Department of Urology, The Second Hospital of Hebei Medical University, China (Z.Y.); and Department of Cardiovascular Sciences, University of Leicester, United Kingdom (T.S.).
Jin-Kun WenFrom the Department of Biochemistry and Molecular Biology, The Key Laboratory of Neural and Vascular Biology, Ministry of Education of China, Hebei Medical University (Y.S., Z.Y., B.Z., X.-h.Z., M.-l.Z., X.-s.Z., H.-y.Z., J.-k.W.); Department of Urology, The Second Hospital of Hebei Medical University, China (Z.Y.); and Department of Cardiovascular Sciences, University of Leicester, United Kingdom (T.S.). wjk@hebmu.edu.cn.
Hebei Medical University · CNSecond Hospital of Hebei Medical University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

rationaleNeuregulin-1 (NRG-1) includes an extracellular epidermal growth factor-like domain and an intracellular domain (NRG-1-ICD). In response to transforming growth factor-β1, its cleavage by proteolytic enzymes releases a bioactive fragment, which suppresses the vascular smooth muscle cell (VSMC) proliferation by activating ErbB (erythroblastic leukemia viral oncogene homolog) receptor. However, NRG-1-ICD function in VSMCs remains unknown.

objectiveHere, we characterize the function of NRG-1-ICD and underlying mechanisms in VSMCs. METHODS AND

resultsImmunofluorescence staining, Western blotting, and quantitative real-time polymerase chain reaction showed that NRG-1 was expressed in rat, mouse, and human VSMCs and was upregulated and cleaved in response to transforming growth factor-β1. In the cytoplasm of HASMCs (human aortic smooth muscle cells), the NRG-1-ICD participated in filamentous actin formation by interacting with α-SMA (smooth muscle α-actin). In the nucleus, the Nrg-1-ICD induced circular ACTA2 (alpha-actin-2; circACTA2) formation by recruitment of the zinc-finger transcription factor IKZF1 (IKAROS family zinc finger 1) to the first intron of α-SMA gene. We further confirmed that circACTA2, acting as a sponge binding microRNA (miR)-548f-5p, interacted with miR-548f-5p targeting 3' untranslated region of α-SMA mRNA, which in turn relieves miR-548f-5p repression of the α-SMA expression and thus upregulates α-SMA expression, thereby facilitating stress fiber formation and cell contraction in HASMCs. Accordingly, in vivo studies demonstrated that the localization of the interaction of circACTA2 with miR-548f-5p is significantly decreased in human intimal hyperplastic arteries compared with normal arteries, implicating that dysregulation of circACTA2 and miR-548f-5p expression is involved in intimal hyperplasia.

conclusionsThese results suggest that circACTA2 mediates NRG-1-ICD regulation of α-SMA expression in HASMCs via the NRG-1-ICD/circACTA2/miR-548f-5p axis. Our data provide a molecular basis for fine-tuning α-SMA expression and VSMC contraction by transcription factor, circular RNA, and microRNA.

Indexed as

ActinsAnimalsCells, CulturedHEK293 CellsHumansIkaros Transcription FactorMiceMice, Inbred C57BLMicroRNAsMuscle, Smooth, VascularMyocytes, Smooth MuscleNeuregulin-1RatsActinsIkaros Transcription FactorMicroRNAsNeuregulin-1ACTA2circular RNAmiR-548neuregulin-1smooth muscle cellsTGF-beta1

Identifiers

PMID28698179
OpenAlexW2735775598

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.