ArticleBiophysical journal2017
Measuring Protein Binding to Lipid Vesicles by Fluorescence Cross-Correlation Spectroscopy.
Article in Biophysical journal, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed.
- Article
- The minimal membrane requirements for BAX-induced pore opening upon exposure to oxidative stress.Biophysical journal · 2024Article
- Dissecting the biophysical mechanisms of oleate hydratase association with membranes.Frontiers in molecular biosciences · 2024Article
- Quantitative characterization of membrane-protein reversible association using FCS.Biophysical journal · 2023Article
- Single-Particle Analysis of the Interaction Between Molecules and Protein Aggregated Species by Dual-Color Time-Resolved Fluorescence Spectroscopy.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Article
- Ionic Strength and Solution Composition Dictate the Adsorption of Cell-Penetrating Peptides onto Phosphatidylcholine Membranes.Langmuir : the ACS journal of surfaces and colloids · 2022Article
- Review
- α-Helical peptidic scaffolds to target α-synuclein toxic species with nanomolar affinity.Nature communications · 2021Article
- Cooperativity of α-Synuclein Binding to Lipid Membranes.ACS chemical neuroscience · 2021Article
- Measuring protein insertion areas in lipid monolayers by fluorescence correlation spectroscopy.Biophysical journal · 2021Article
- High-throughput fluorescence correlation spectroscopy enables analysis of surface components of cell-derived vesicles.Analytical and bioanalytical chemistry · 2020Article
- Giant Endoplasmic Reticulum vesicles (GERVs), a novel model membrane tool.Scientific reports · 2020Article
- Organelle-specific targeting of polymersomes into the cell nucleus.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Navigating the Landscape of Tumor Extracellular Vesicle Heterogeneity.International journal of molecular sciences · 2019Review
- Article
Corrections and comments
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Authors and funding
4 authors.
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No grant is acknowledged in the PubMed record.
Abstract
Fluorescence correlation spectroscopy has been previously used to investigate peptide and protein binding to lipid membranes, as it allows for very low amounts of sample, short measurement times and equilibrium binding conditions. Labeling only one of the binding partners, however, comes with certain drawbacks, as it relies on identifying binding events by a change in diffusion coefficient. Since peptide and protein aggregation can obscure specific binding, and since non-stoichiometric binding necessitates the explicit choice of a statistical distribution for the number of bound ligands, we additionally label the liposomes and perform dual-color fluorescence cross-correlation spectroscopy (dcFCCS). We develop a theoretical framework showing that dcFCCS amplitudes allow calculation of the degree of ligand binding and the concentration of unbound ligand, leading to a model-independent binding curve. As the degree of labeling of the ligands does not factor into the measured quantities, it is permissible to mix labeled and unlabeled ligand, thereby extending the range of usable protein concentrations and accessible dissociation constants, K
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