ArticleDevelopmental cell2017
A Tubulin Binding Switch Underlies Kip3/Kinesin-8 Depolymerase Activity.
Article in Developmental cell, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.
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Who cites it
41 citing papers in PubMed, 80 citations in OpenAlex.
- Distinct disassembly modes shape cytoskeletal assembly and fluctuations.Biophysical reports · 2026Article
- Quantitative Signatures of Disassembly Mechanisms Modulating Filament and Bundle Assembly in a Shared Pool.bioRxiv : the preprint server for biology · 2025Article
- Mechanism and regulation of kinesin motors.Nature reviews. Molecular cell biology · 2025Review
- Phase separation of microtubule-binding proteins - implications for neuronal function and disease.Journal of cell science · 2024Review
- Modeling study of kinesin-13 MCAK microtubule depolymerase.European biophysics journal : EBJ · 2024Article
- Article
- Meiosis II spindle disassembly requires two distinct pathways.Molecular biology of the cell · 2023Article
- New insights into the mechanochemical coupling mechanism of kinesin-microtubule complexes from their high-resolution structures.Biochemical Society transactions · 2023Review
- The motor domain of the kinesin Kip2 promotes microtubule polymerization at microtubule tips.The Journal of cell biology · 2023Article
- Determinant factors for residence time of kinesin motors at microtubule ends.Journal of biological physics · 2023Article
- The effect of motor-induced shaft dynamics on microtubule stability and length.Biophysical journal · 2023Article
- Mechanochemical tuning of a kinesin motor essential for malaria parasite transmission.Nature communications · 2022Article
- A synthetic ancestral kinesin-13 depolymerizes microtubules faster than any natural depolymerizing kinesin.Open biology · 2022Article
- Kinesin-8-specific loop-2 controls the dual activities of the motor domain according to tubulin protofilament shape.Nature communications · 2022Article
- Microtubule dynamics influence the retrograde biased motility of kinesin-4 motor teams in neuronal dendrites.Molecular biology of the cell · 2022Article
- Atomic force microscopy reveals distinct protofilament-scale structural dynamics in depolymerizing microtubule arrays.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Reconstituting Microtubules: A Decades-Long Effort From Building Block Identification to the Generation of Recombinant α/β-Tubulin.Frontiers in cell and developmental biology · 2022Review
- Regulation of microtubule dynamics, mechanics and function through the growing tip.Nature reviews. Molecular cell biology · 2021Review
- Epistasis, aneuploidy, and functional mutations underlie evolution of resistance to induced microtubule depolymerization.The EMBO journal · 2021Article
- A model of processive walking and slipping of kinesin-8 molecular motors.Scientific reports · 2021Article
Corrections and comments
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Authors and funding
8 authors at 6 institutions in 1 country.
Funding
Abstract
Kinesin-8 motors regulate the size of microtubule structures, using length-dependent accumulation at the plus end to preferentially disassemble long microtubules. Despite extensive study, the kinesin-8 depolymerase mechanism remains under debate. Here, we provide evidence for an alternative, tubulin curvature-sensing model of microtubule depolymerization by the budding yeast kinesin-8, Kip3. Kinesin-8/Kip3 uses ATP hydrolysis, like other kinesins, for stepping on the microtubule lattice, but at the plus end Kip3 undergoes a switch: its ATPase activity is suppressed when it binds tightly to the curved conformation of tubulin. This prolongs plus-end binding, stabilizes protofilament curvature, and ultimately promotes microtubule disassembly. The tubulin curvature-sensing model is supported by our identification of Kip3 structural elements necessary and sufficient for plus-end binding and depolymerase activity, as well as by the identification of an α-tubulin residue specifically required for the Kip3-curved tubulin interaction. Together, these findings elucidate a major regulatory mechanism controlling the size of cellular microtubule structures.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.