ArticleScientific data2017
A multi-protease, multi-dissociation, bottom-up-to-top-down proteomic view of the Loxosceles intermedia venom.
Article in Scientific data, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed.
- Proteome Analysis of the Six-Eyed Sand-SpiderToxins · 2025Article
- Comparative Biochemical, Structural, and Functional Analysis of Recombinant Phospholipases D from ThreeInternational journal of molecular sciences · 2023Article
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- Toxinology in the proteomics era: a review on arachnid venom proteomics.The journal of venomous animals and toxins including tropical diseases · 2022Article
- Mass spectrometry-based top-down and bottom-up approaches for proteomic analysis of the Moroccan Buthus occitanus scorpion venom.FEBS open bio · 2021Article
- Brown spider venom toxins: what are the functions of astacins, serine proteases, hyaluronidases, allergens, TCTP, serpins and knottins?The journal of venomous animals and toxins including tropical diseases · 2021Article
- Prospective Use of Brown Spider Venom Toxins as Therapeutic and Biotechnological Inputs.Frontiers in molecular biosciences · 2021Review
- Flying blind, or just flying under the radar? The underappreciated power of de novo methods of mass spectrometric peptide identification.Protein science : a publication of the Protein Society · 2020Review
- The Power of Three in Cannabis Shotgun Proteomics: Proteases, Databases and Search Engines.Proteomes · 2020Article
- Review
- TCTP fromCells · 2019Article
- A Multiple Protease Strategy to Optimise the Shotgun Proteomics of Mature Medicinal Cannabis Buds.International journal of molecular sciences · 2019Article
- Spider Venom: Components, Modes of Action, and Novel Strategies in Transcriptomic and Proteomic Analyses.Toxins · 2019Review
- Brown Spider (Toxins · 2019Review
- The Role of Electron Transfer Dissociation in Modern Proteomics.Analytical chemistry · 2018Review
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Authors and funding
11 authors.
Funding
Abstract
Venoms are a rich source for the discovery of molecules with biotechnological applications, but their analysis is challenging even for state-of-the-art proteomics. Here we report on a large-scale proteomic assessment of the venom of Loxosceles intermedia, the so-called brown spider. Venom was extracted from 200 spiders and fractioned into two aliquots relative to a 10 kDa cutoff mass. Each of these was further fractioned and digested with trypsin (4 h), trypsin (18 h), pepsin (18 h), and chymotrypsin (18 h), then analyzed by MudPIT on an LTQ-Orbitrap XL ETD mass spectrometer fragmenting precursors by CID, HCD, and ETD. Aliquots of undigested samples were also analyzed. Our experimental design allowed us to apply spectral networks, thus enabling us to obtain meta-contig assemblies, and consequently de novo sequencing of practically complete proteins, culminating in a deep proteome assessment of the venom. Data are available via ProteomeXchange, with identifier PXD005523.
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