ArticleMarine biotechnology (New York, N.Y.)2017
Recovery of Previously Uncultured Bacterial Genera from Three Mediterranean Sponges.
Article in Marine biotechnology (New York, N.Y.), 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 18 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
18 citing papers in PubMed.
- Pseudovibriamide B from marine sponge-associated bacteria acts as a selective antibiotic antidote.ISME communications · 2026Article
- Biodiversity and Biological Interactions of Actinobacteria Associated with Deep Sea and Intertidal Marine Invertebrates.Marine drugs · 2025Article
- A metabologenomics approach to unlock the metabolome of the novel Antarctic deep-sea isolatePNAS nexus · 2023Article
- Comparative Genomic Analysis ofMicroorganisms · 2023Article
- Genomic Analysis ofToxins · 2022Article
- Selective isolation of Arctic marine actinobacteria and a down-scaled fermentation and extraction strategy for identifying bioactive compounds.Frontiers in microbiology · 2022Article
- Article
- Bioactivity Screening and Gene-Trait Matching across Marine Sponge-Associated Bacteria.Marine drugs · 2021Article
- Bacteria Cultivated From Sponges and Bacteria Not Yet Cultivated From Sponges-A Review.Frontiers in microbiology · 2021Review
- Comparative genomic analysis of Flavobacteriaceae: insights into carbohydrate metabolism, gliding motility and secondary metabolite biosynthesis.BMC genomics · 2020Article
- Phylogenomic Analyses of Members of the Widespread Marine Heterotrophic GenusApplied and environmental microbiology · 2020Article
- Cultivation of Bacteria FromFrontiers in microbiology · 2020Article
- Cultivation of Sponge-Associated Bacteria fromMarine drugs · 2019Article
- Chemical Ecology of Marine Sponges: New Opportunities through "-Omics".Integrative and comparative biology · 2019Review
- Genomic blueprints of sponge-prokaryote symbiosis are shared by low abundant and cultivatable Alphaproteobacteria.Scientific reports · 2019Article
- Ecology and Biotechnological Potential of Bacteria Belonging to the Genus Pseudovibrio.Applied and environmental microbiology · 2018Review
- Comparative Genomics Highlights Symbiotic Capacities and High Metabolic Flexibility of the Marine Genus Pseudovibrio.Genome biology and evolution · 2018Article
- First Molecular Identification of Symbiotic Archaea in a Sponge Collected from the Persian Gulf, Iran.The open microbiology journal · 2018Article
Corrections and comments
- Erratum issued
Authors and funding
5 authors.
Funding
Abstract
Sponges often harbour a dense and diverse microbial community. Presently, a large discrepancy exists between the cultivable bacterial fraction from sponges and the community in its natural environment. Here, we aimed to acquire additional insights into cultivability of (previously uncultured) bacteria from three sponge species, namely Aplysina aerophoba, Corticium candelabrum and Petrosia ficiformis, by studying bacterial growth on five media in the form of 60 communities scraped from plates without antibiotics, as well as in the form of individual isolates that were grown on these media supplemented with antibiotics. We applied (double-)barcoded 16S ribosomal RNA (rRNA) gene amplicon sequencing for species identification. We show that previously uncultured bacteria can be cultivated using conventional plating and that application of antibiotics in the media can serve to capture a greater bacterial diversity. Moreover, we present criteria to address an important caveat of the plate scraping method whereby bacteria may be detected that did not actually grow. Fourteen out of 27 cultivated novel taxa (<95% identity of the 16S rRNA gene amplicon to reported species) belong to Actinobacteria, which indicates the presence of a large untapped reservoir of bioactive compounds. Three Flavobacteriaceae spp. were isolated that potentially constitute two new genera and one new species.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.