ArticleZoological letters2017
An efficient system for homology-dependent targeted gene integration in medaka (
Article in Zoological letters, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 12 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
12 citing papers in PubMed, 38 citations in OpenAlex.
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- Ripply suppresses Tbx6 to induce dynamic-to-static conversion in somite segmentation.Nature communications · 2023Article
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- Speciation and adaptation research meets genome editing.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2022Review
- CRISPR/Cas9-mediated knock-in of alligator cathelicidin gene in a non-coding region of channel catfish genome.Scientific reports · 2020Article
- Indel locations are determined by template polarity in highly efficient in vivo CRISPR/Cas9-mediated HDR in Atlantic salmon.Scientific reports · 2020Article
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- Medaka-microinjection with an Upright Microscope.Bio-protocol · 2018Article
- Highly efficient generation of knock-in transgenic medaka by CRISPR/Cas9-mediated genome engineering.Zoological letters · 2018Article
- Genotyping-free Selection of Double Allelic Gene Edited Medaka Using Two Different Fluorescent Proteins.Bio-protocol · 2017Article
- Correction to: An efficient system for homology-dependent targeted gene integration in medaka (Zoological lettersArticle
Corrections and comments
- Erratum issued
Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe CRISPR/Cas system is a powerful genome editing tool that enables targeted genome modifications in various organisms. In medaka (
resultsWe show that longer homology arms (ca. 500 bp) and linearization of circular donor plasmids by cleavage with bait sequences enhances the efficiency of targeted integration of plasmids in embryos. A new bait sequence, BaitD, which we designed and selected by in silico screening, achieved the highest efficiency of the targeted gene integration in vivo. Using this system, donor plasmids integrated precisely at target sites and were efficiently transmitted to progeny. We also report that the genotype of F
conclusionWe report that the efficiency of targeted gene integration can be enhanced by using donor vectors with longer homologous arms and linearization using a highly active bait system in medaka. These findings may contribute to the establishment of more efficient systems for targeted gene integration in medaka and other fish species.
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