Evidence map›Paper›PMID 28694659›Full record

ArticleWorld journal of gastroenterology2017

Induction of chronic cholestasis without liver cirrhosis - Creation of an animal model.

Felix Dondorf, René Fahrner, Michael Ardelt, Eleonora Patsenker, Felix Stickel, Uta Dahmen, Utz Settmacher, Falk Rauchfuß

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in World journal of gastroenterology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.9field-weighted citation impact, top 27% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 9 citations in OpenAlex.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 3 institutions in 2 countries.

Felix DondorfFelix Dondorf, René Fahrner, Michael Ardelt, Uta Dahmen, Utz Settmacher, Falk Rauchfuß, Department of General, Visceral and Vascular Surgery, Jena University Hospital, 07747 Jena, Germany.
René FahrnerFelix Dondorf, René Fahrner, Michael Ardelt, Uta Dahmen, Utz Settmacher, Falk Rauchfuß, Department of General, Visceral and Vascular Surgery, Jena University Hospital, 07747 Jena, Germany.
Michael ArdeltFelix Dondorf, René Fahrner, Michael Ardelt, Uta Dahmen, Utz Settmacher, Falk Rauchfuß, Department of General, Visceral and Vascular Surgery, Jena University Hospital, 07747 Jena, Germany.
Eleonora PatsenkerFelix Dondorf, René Fahrner, Michael Ardelt, Uta Dahmen, Utz Settmacher, Falk Rauchfuß, Department of General, Visceral and Vascular Surgery, Jena University Hospital, 07747 Jena, Germany.
Felix StickelFelix Dondorf, René Fahrner, Michael Ardelt, Uta Dahmen, Utz Settmacher, Falk Rauchfuß, Department of General, Visceral and Vascular Surgery, Jena University Hospital, 07747 Jena, Germany.
Uta DahmenFelix Dondorf, René Fahrner, Michael Ardelt, Uta Dahmen, Utz Settmacher, Falk Rauchfuß, Department of General, Visceral and Vascular Surgery, Jena University Hospital, 07747 Jena, Germany.
Utz SettmacherFelix Dondorf, René Fahrner, Michael Ardelt, Uta Dahmen, Utz Settmacher, Falk Rauchfuß, Department of General, Visceral and Vascular Surgery, Jena University Hospital, 07747 Jena, Germany.
Falk RauchfußFelix Dondorf, René Fahrner, Michael Ardelt, Uta Dahmen, Utz Settmacher, Falk Rauchfuß, Department of General, Visceral and Vascular Surgery, Jena University Hospital, 07747 Jena, Germany.
Jena University Hospital · DEUniversity of Zurich · CHUniversity Hospital of Zurich · CH

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

aimTo analyze time intervals of inflammation and regeneration in a cholestatic rat liver model.

methodsIn 36 Lewis rats, divided into six groups of 6 animals (postoperative observation periods: 1, 2, 3, 4, 6, 8 wk), the main bile duct was ligated with two ligatures and observed for the periods mentioned above. For laboratory evaluation, cholestasis parameters (bilirubin, γ-GT), liver cell parameters (ASAT, ALAT) and liver synthesis parameters (quick, albumin) were determined. For histological analysis, HE, EvG, ASDCL and HMGB-1 stainings were performed. Furthermore, we used the mRNA of IL-33, GADD45a and p-21 for analyzing cellular stress and regeneration in cholestatic rats.

resultsIn chemical laboratory and histological evaluation, a distinction between acute and chronic cholestatic liver injury with identification of inflammation and regeneration could be demonstrated by an increase in cholestasis (bilirubin: 1-wk group, 156.83 ± 34.12 μmol/L,

conclusionWe created a chronic cholestasis model. The point of inflammatory and regenerative balance was reached after four weeks. This finding should be used for experimental approaches dealing with chronic cholestatic liver damage.

Indexed as

Disease Models, AnimalRegenerationAnimalsBile DuctsBody WeightCell Cycle ProteinsCholestasisCyclin-Dependent Kinase Inhibitor p21GADD45 ProteinsHumansInflammationInterleukin-33LiverLiver CirrhosisLiver Cirrhosis, BiliaryMaleCdkn1a protein, ratCell Cycle ProteinsCyclin-Dependent Kinase Inhibitor p21Gadd45a protein, ratGADD45 ProteinsIl33 protein, ratInterleukin-33Nuclear ProteinsRNA, MessengerCholestasisChronic cholestasisRat liver modelRats

Identifiers

PMID28694659
PMCPMC5483493
OpenAlexW2695145975

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.