ReviewBioscience reports2017
Single-molecule fluorescence microscopy review: shedding new light on old problems.
Review in Bioscience reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 114 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
114 citing papers in PubMed.
- Concurrent positional dynamics and activity mapping of DNA-binding proteins.Nature protocols · 2026Review
- Explainable deep learning framework for fecal contamination detection on chicken eggshells via portable fluorescence imaging under ambient light.Poultry science · 2026Article
- Single-Molecule Detection Concepts Enabled by DNA Origami.Micromachines · 2026Review
- Metal-Enhanced Fluorescence for the Biologist's Cellular Imaging Toolkit: Design Principles and Recent Applications.BioEssays : news and reviews in molecular, cellular and developmental biology · 2026Review
- Circularization and Ribosome Recycling: From Polysome Topology to Translational Control.International journal of molecular sciences · 2026Review
- The transitional kinetics between open and closed Rep structures can be tuned by salt via two intermediate states.Nucleic acids research · 2026Article
- Localization of Proteins in the Secretory Pathway of Pichia pastoris Using Fluorescence Microscopy.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Fluorescent nanoplastics: What steps are needed towards a representative toolkit?Microplastics and nanoplastics · 2026Review
- Breath by Breath: Navigating the Landscape of Exhaled Breath in Clinical Research and Prospects for the Future.Journal of analytical methods in chemistry · 2026Review
- HDCluster: High-Degree Graph Clustering for Robust Analysis of Single Molecule Localization Microscopy.bioRxiv : the preprint server for biology · 2025Article
- Review
- Fluorescence of Atomic Germanium - Solution of Excitation Transfer.Journal of fluorescence · 2025Article
- Foundation model for efficient biological discovery in single-molecule time traces.Nature methods · 2025Article
- Signal Amplification for Fluorescent Staining of Single Particles in Liquid Biopsies: Circulating Tumour Cells and Extracellular Vesicles.Journal of extracellular vesicles · 2025Article
- Label-Free Single-Molecule Immunoassay.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Single-Molecule Fluorescence Imaging of Energy-Related Catalytic Reactions.Chemical & biomedical imaging · 2025Review
- Advancements in Single-Molecule Fluorescence Detection Techniques and Their Expansive Applications in Drug Discovery and Neuroscience.Biosensors · 2025Review
- Fluorogenic Linkage Integration for Nonfluorescent Transformations (FLINT).Chemical & biomedical imaging · 2025Article
- Wash-free fluorescent tools based on organic molecules: Design principles and biomedical applications.Exploration (Beijing, China) · 2025Review
- Pretrained Deep Neural Network Kin-SiM for Single-Molecule FRET Trace Idealization.The journal of physical chemistry. B · 2025Article
54 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors.
Funding
Abstract
Fluorescence microscopy is an invaluable tool in the biosciences, a genuine workhorse technique offering exceptional contrast in conjunction with high specificity of labelling with relatively minimal perturbation to biological samples compared with many competing biophysical techniques. Improvements in detector and dye technologies coupled to advances in image analysis methods have fuelled recent development towards single-molecule fluorescence microscopy, which can utilize light microscopy tools to enable the faithful detection and analysis of single fluorescent molecules used as reporter tags in biological samples. For example, the discovery of GFP, initiating the so-called 'green revolution', has pushed experimental tools in the biosciences to a completely new level of functional imaging of living samples, culminating in single fluorescent protein molecule detection. Today, fluorescence microscopy is an indispensable tool in single-molecule investigations, providing a high signal-to-noise ratio for visualization while still retaining the key features in the physiological context of native biological systems. In this review, we discuss some of the recent discoveries in the life sciences which have been enabled using single-molecule fluorescence microscopy, paying particular attention to the so-called 'super-resolution' fluorescence microscopy techniques in live cells, which are at the cutting-edge of these methods. In particular, how these tools can reveal new insights into long-standing puzzles in biology: old problems, which have been impossible to tackle using other more traditional tools until the emergence of new single-molecule fluorescence microscopy techniques.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.