ArticleMicrobial cell factories2017
Characterization of erythrose reductase from Yarrowia lipolytica and its influence on erythritol synthesis.
Article in Microbial cell factories, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 81 citations in OpenAlex.
- Elucidating the role of YlMDH2 in temperature-dependent mannitol biosynthesis in Yarrowia lipolytica via integrative multi-omics and structural analysis.International microbiology : the official journal of the Spanish Society for Microbiology · 2026Article
- Engineered yeast Yarrowia lipolytica as a chassis for biosynthesis of fatty acids from mannitol and macroalgal biomass extracts.Microbial cell factories · 2025Article
- Protein rational design and modification of erythrose reductase for the improvement of erythritol production in Yarrowia lipolytica.Bioprocess and biosystems engineering · 2024Article
- Research progress on biosynthesis of erythritol and multi-dimensional optimization of production strategies.World journal of microbiology & biotechnology · 2024Review
- Application of NewInternational journal of molecular sciences · 2024Article
- Comprehensive phenomic and genomic studies of the species,Frontiers in plant science · 2024Article
- Multiplex modification of Yarrowia lipolytica for enhanced erythritol biosynthesis from glycerol through modularized metabolic engineering.Bioprocess and biosystems engineering · 2023Article
- Understanding the role of GRE3 in the erythritol biosynthesis pathway in Saccharomyces uvarum and its implication in osmoregulation and redox homeostasis.Microbial biotechnology · 2023Article
- In-depth analysis of erythrose reductase homologs in Yarrowia lipolytica.Scientific reports · 2023Article
- Enhancing the thermotolerance and erythritol production ofFrontiers in bioengineering and biotechnology · 2023Article
- "Fight-flight-or-freeze" - how Yarrowia lipolytica responds to stress at molecular level?Applied microbiology and biotechnology · 2022Review
- Enhancing erythritol production from crude glycerol in a wild-typeFrontiers in microbiology · 2022Article
- The Overexpression ofMolecules (Basel, Switzerland) · 2021Article
- High value-added products derived from crude glycerol via microbial fermentation using Yarrowia clade yeast.Microbial cell factories · 2021Article
- The Role of Hexokinase and Hexose Transporters in Preferential Use of Glucose over Fructose and Downstream Metabolic Pathways in the YeastInternational journal of molecular sciences · 2021Article
- From the culture broth to the erythritol crystals: an opportunity for circular economy.Applied microbiology and biotechnology · 2021Review
- Recent Advances in Producing Sugar Alcohols and Functional Sugars by EngineeringFrontiers in bioengineering and biotechnology · 2021Review
- Article
- Nitrogen as the major factor influencing gene expression inBiotechnology reports (Amsterdam, Netherlands) · 2020Article
- Sugar Alcohols and Organic Acids Synthesis inMicroorganisms · 2020Review
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundErythritol is a natural sweetener that is used in the food industry. It is produced as an osmoprotectant by bacteria and yeast. Due to its chemical properties, it does not change the insulin level in the blood, and therefore it can be safely used by diabetics. Previously, it has been shown that erythrose reductase (ER), which catalyzes the final step, plays a crucial role in erythritol synthesis. ER reduces erythrose to erythritol with NAD(P)H as a cofactor. Despite many studies on erythritol synthesis by Yarrowia lipolytica, the enzymes involved in this metabolic pathway have ever been described.
resultsThe gene YALI0F18590g encoding the predicted erythrose reductase from Y. lipolytica was overexpressed, and its influence on erythritol synthesis was studied. The amino acid sequence of the Y. lipolytica ER showed a high degree of similarity to the previously described erythrose reductases from known erythritol producers, such as Candida magnoliae and Moniliella megachiliensis. Here, we found that the gene overexpression results in an enhanced titer of erythritol of 44.44 g/L (20% over the control), a yield of 0.44 g/g and productivity of 0.77 g/L/h. Moreover, on purification and characterization of the enzyme we found that it displays the highest activity at 37 °C and pH 3.0. The effects of various metal ions (Zn
conclusionsIn this study we identified, overexpressed and characterized a native erythrose reductase in Y. lipolytica. Further optimizations of this strain via metabolic pathway engineering and media optimization strategies enabled 54 g/L to be produced in a shake-flask experiment. To date, this is the first reported study employing metabolic engineering of the native gene involved in the erythritol pathway to result in a high titer of the polyol. Moreover, it indicates the importance of environmental conditions for genetic targets in metabolic engineering.
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