ArticleAnnals of clinical microbiology and antimicrobials2017
Comparison of in-house and commercial real time-PCR based carbapenemase gene detection methods in Enterobacteriaceae and non-fermenting gram-negative bacterial isolates.
Article in Annals of clinical microbiology and antimicrobials, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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19 citing papers in PubMed, 22 citations in OpenAlex.
- Multidrug-resistant bacterial contamination of healthcare workers' mobile phones, decontamination efficacy, and potential implications for hospital-acquired infections.Scientific reports · 2026Article
- Emergence and Spread of Carbapenem Antimicrobial Resistance: A Review on Mechanism of Drug Resistance and Laboratory Detection.Annals of African medicine · 2026Review
- High-Resolution Melting Curve Analysis (HRMA) for the Identification of Class D β-Lactamases (CHDLs) inInfection and drug resistance · 2026Article
- Bacterial burden and drug-resistant bacteria in healthcare workers' mobile phones: a study in Puerto Rican outpatient clinics.Infection prevention in practice · 2025Article
- Genotypic Characterisation of Carbapenem-Resistant Enterobacteriaceae in a Tertiary Care Hospital in South India.Cureus · 2024Article
- Development and Validation of a Novel Multiplex Real-Time PCR Assay for Rapid Detection of Carbapenemase Genes in Carbapenem-ResistantInfection and drug resistance · 2024Article
- A Review of Carbapenem Resistance inMicroorganisms · 2023Review
- Ten simple rules for the sharing of bacterial genotype-Phenotype data on antimicrobial resistance.PLoS computational biology · 2023Article
- Multiplex PCR Detection of Common Carbapenemase Genes and Identification of Clinically RelevantAntibiotics (Basel, Switzerland) · 2022Article
- Development of Microfluidic Chip-Based Loop-Mediated Isothermal Amplification (LAMP) Method for Detection of Carbapenemase Producing Bacteria.Microbiology spectrum · 2022Article
- Rapid Detection of Beta-Lactamases Genes amongBioMed research international · 2022Article
- Optimization and development of high-resolution melting curve analysis (HRMA) assay for detection of New Delhi metallo-β-lactamase (NDM) producingAIMS microbiology · 2022Article
- In-Vitro Efficacy of Cefiderocol in Carbapenem-Non-Susceptible Gram-Negative Bacilli of Different Genotypes in Sub-Region of North Rhine Westphalia, Germany.Pathogens (Basel, Switzerland) · 2021Article
- Multiplex Real-Time PCR Assay for Six Major Carbapenemase Genes.Pathogens (Basel, Switzerland) · 2021Article
- Synergistic Activity of Equol and Meropenem against Carbapenem-ResistantAntibiotics (Basel, Switzerland) · 2021Article
- Current state of the art in rapid diagnostics for antimicrobial resistance.Lab on a chip · 2020Review
- Screening for Carbapenemases in Ertapenem-ResistantEuropean journal of microbiology & immunology · 2019Article
- Screening for carriage of carbapenem-resistant Enterobacteriaceae in settings of high endemicity: a position paper from an Italian working group on CRE infections.Antimicrobial resistance and infection control · 2019Review
- Prevalence ofIranian journal of pathology · 2018Article
Corrections and comments
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Authors and funding
4 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundCarbapenemase-producing gram-negative bacteria are increasing globally and have been associated with outbreaks in hospital settings. Thus, the accurate detection of these bacteria in infections is mandatory for administering the adequate therapy and infection control measures. This study aimed to establish and evaluate a multiplex real-time PCR assay for the simultaneous detection of carbapenemase gene variants in gram-negative rods and to compare the performance with a commercial RT-PCR assay (Check-Direct CPE).
methods116 carbapenem-resistant Enterobacteriaceae, Pseudomonas aeruginosa and Acinetobacter baumannii isolates were genotyped for carbapenemase genes by PCR and sequencing. The defined isolates were used for the validation of the in-house RT-PCR by use of designed primer pairs and probes.
resultsAmong the carbapenem-resistant isolates the genes bla
conclusionThe Check-Direct CPE and the in-house RT-PCR assay detected the carbapenem resistance from solid culture isolates. Moreover, the in-house assay enabled the identification of carbapenemase genes directly from positive blood-culture vials. However, we observed insufficient detection of various OXA genes in both assays. Nevertheless, the in-house RT-PCR detected the majority of the OXA type genes in Enterobacteriaceae and A. baumannii.
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