Evidence map›Paper›PMID 28693424›Full record

ArticleBMC microbiology2017

Bacillus subtilis IolQ (DegA) is a transcriptional repressor of iolX encoding NAD

Dong-Min Kang, Christophe Michon, Tetsuro Morinaga, Kosei Tanaka, Shinji Takenaka, Shu Ishikawa, Ken-Ichi Yoshida

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Article in BMC microbiology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Article
  2. TheJournal of bacteriology · 2019
    Article
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Dong-Min KangDepartment of Agrobioscience, Graduate School of Agricultural Science, Kobe University, 1-1 Rokkodai, Nada, Kobe, 657-8501, Japan.
Christophe MichonDepartment of Science, Technology and Innovation, Graduate School of Science, Technology and Innovation, Kobe University, 1-1 Rokkodai, Nada, Kobe, 657-8501, Japan.
Tetsuro MorinagaGene testing Business Department, LS Business Division, Sysmex Corporation, 4-4-4 Takatsukadai, Nishi, Kobe, 651-2271, Japan.
Kosei TanakaOrganization of Advanced Science and Technology, Kobe University, 1-1 Rokkodai, Nada, Kobe657, Kobe, -8501, Japan.
Shinji TakenakaDepartment of Agrobioscience, Graduate School of Agricultural Science, Kobe University, 1-1 Rokkodai, Nada, Kobe, 657-8501, Japan.
Shu IshikawaDepartment of Science, Technology and Innovation, Graduate School of Science, Technology and Innovation, Kobe University, 1-1 Rokkodai, Nada, Kobe, 657-8501, Japan.
Ken-Ichi YoshidaOrganization of Advanced Science and Technology, Kobe University, 1-1 Rokkodai, Nada, Kobe657, Kobe, -8501, Japan. kenyoshi@kobe-u.ac.jp.ORCID http://orcid.org/0000-0002-3383-4664

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundBacillus subtilis is able to utilize at least three inositol stereoisomers as carbon sources, myo-, scyllo-, and D-chiro-inositol (MI, SI, and DCI, respectively). NAD

resultsImmediately upstream of iolX, there is an operon that encodes two genes, yisR and iolQ (formerly known as degA), each of which could encode a transcriptional regulator. Here we performed an inactivation analysis of yisR and iolQ and found that iolQ encodes a repressor of the iolX transcription. The coding sequence of iolQ was expressed in Escherichia coli and the gene product was purified as a His-tagged fusion protein, which bound to two sites within the iolX promoter region in vitro.

conclusionsIolQ is a transcriptional repressor of iolX. Genetic evidences allowed us to speculate that SI and MI might possibly be the intracellular inducers, however they failed to antagonize DNA binding of IolQ in in vitro experiments.

Indexed as

Gene Expression Regulation, EnzymologicBacillus subtilisBacterial ProteinsGene Expression Regulation, BacterialInositolNADPromoter Regions, GeneticRepressor ProteinsSugar Alcohol DehydrogenasesBacterial ProteinsDegA protein, Bacillus subtilisInositolIolX protein, Bacillus subtilisNADRepressor ProteinsscyllitolSugar Alcohol DehydrogenasesBacillus subtilisInositol dehydrogenaseRepressorscyllo-inositolTranscription

Identifiers

PMID28693424
PMCPMC5504672

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.