ArticleBMC genetics2017
De novo transcriptomic analysis of cowpea (Vigna unguiculata L. Walp.) for genic SSR marker development.
Article in BMC genetics, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed.
- Application of Omics Technologies for Cowpea Improvement.Plant-environment interactions (Hoboken, N.J.) · 2026Review
- RNA-Seq-based transcriptomics reveals differential gene expression between two Pisum sativum subspecies and uncovers their molecular marker profiles.BMC genomics · 2025Article
- Exogenous application of γ-aminobutyric acid alleviates temperature stress in mungbean (Vigna radiata) and its wild non-progenitor (Vigna glabrescens) by regulating heat shock protein genes.BMC plant biology · 2025Article
- Stage-Specific Transcriptomic Insights into Seed Germination and Early Development inPlants (Basel, Switzerland) · 2025Article
- Genetic diversity and population structure of cowpea mutant collection using SSR and ISSR molecular markers.Scientific reports · 2024Article
- De Novo Transcriptome Assembly of Rice Bean (Plants (Basel, Switzerland) · 2024Article
- An Integrated Approach for Biofortification of Carotenoids in Cowpea for Human Nutrition and Health.Plants (Basel, Switzerland) · 2024Review
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- Deficiencies in the Risk Assessment of Genetically Engineered Bt Cowpea Approved for Cultivation in Nigeria: A Critical Review.Plants (Basel, Switzerland) · 2022Review
- Chemical and molecular examinations of some cowpea genotypes using simple sequence repeat and intersimple sequence repeat DNA markers in relation to their cooking quality.Food science & nutrition · 2021Article
- Applications of Multi-Omics Technologies for Crop Improvement.Frontiers in plant science · 2021Review
- Transcriptome Analysis Reveals Key Pathways and Candidate Genes Controlling Seed Development and Size in Ricebean (Frontiers in genetics · 2021Article
- Constraints and Prospects of Improving Cowpea Productivity to Ensure Food, Nutritional Security and Environmental Sustainability.Frontiers in plant science · 2021Review
- De novo assembly, transcriptome characterization, and simple sequence repeat marker development in duckweedPhysiology and molecular biology of plants : an international journal of functional plant biology · 2020Article
- New EST-SSR Markers for Individual Genotyping of Opium Poppy Cultivars (Plants (Basel, Switzerland) · 2019Article
- Article
- VigSatDB: genome-wide microsatellite DNA marker database of three species of Vigna for germplasm characterization and improvement.Database : the journal of biological databases and curation · 2019Article
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundCowpea [Vigna unguiculata (L.) Walp.] is one of the most important legumes in tropical and semi-arid regions. However, there is relatively little genomic information available for genetic research on and breeding of cowpea. The objectives of this study were to analyse the cowpea transcriptome and develop genic molecular markers for future genetic studies of this genus.
resultsApproximately 54 million high-quality cDNA sequence reads were obtained from cowpea based on Illumina paired-end sequencing technology and were de novo assembled to generate 47,899 unigenes with an N50 length of 1534 bp. Sequence similarity analysis revealed 36,289 unigenes (75.8%) with significant similarity to known proteins in the non-redundant (Nr) protein database, 23,471 unigenes (49.0%) with BLAST hits in the Swiss-Prot database, and 20,654 unigenes (43.1%) with high similarity in the Kyoto Encyclopedia of Genes and Genomes (KEGG) database. Further analysis identified 5560 simple sequence repeats (SSRs) as potential genic molecular markers. Validating a random set of 500 SSR markers yielded 54 polymorphic markers among 32 cowpea accessions.
conclusionsThis transcriptomic analysis of cowpea provided a valuable set of genomic data for characterizing genes with important agronomic traits in Vigna unguiculata and a new set of genic SSR markers for further genetic studies and breeding in cowpea and related Vigna species.
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