ArticleRNA biology2017
One-step hot formamide extraction of RNA from Saccharomyces cerevisiae.
Article in RNA biology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
36 citing papers in PubMed, 60 citations in OpenAlex.
- A Trizol-Beadbeater RNA extraction method for yeast, and comparison to Novogene RNA extraction.bioRxiv : the preprint server for biology · 2025Article
- Precise measurement of molecular phenotypes with barcode-based CRISPRi systems.Genome biology · 2025Article
- Crucial roles of Grr1 in splicing and translation of HAC1 mRNA upon unfolded stress response.Nature communications · 2025Article
- Rpl12 is a conserved ribophagy receptor.Nature cell biology · 2025Article
- Mitochondrial mRNA and the small subunit rRNA in budding yeasts undergo 3'-end processing at conserved species-specific elements.RNA (New York, N.Y.) · 2025Article
- Modified pea apyrase has altered nuclear functions and enhances the growth of yeast and Arabidopsis.Frontiers in plant science · 2025Article
- The CDK8 kinase module: A novel player in the transcription of translation initiation and ribosomal genes.Molecular biology of the cell · 2025Article
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- Structure-guided discovery of ancestral CRISPR-Cas13 ribonucleases.Science (New York, N.Y.) · 2024Article
- Interplay of the Tfb1 pleckstrin homology domain with Rad2 and Rad4 in transcription coupled and global genomic nucleotide excision repair.Nucleic acids research · 2024Article
- Detection of ribophagy in yeast and mammals.Biophysics reports · 2024Article
- Eukaryotic RNA-guided endonucleases evolved from a unique clade of bacterial enzymes.Nucleic acids research · 2023Article
- [Functional peptidomics analysis ofSe pu = Chinese journal of chromatography · 2023Article
- Composition of the Solvation Shell of the Selected Cyclic Ethers (1,4-Dioxane, 12-Crown-4, 15-Crown-5 and 18-Crown-6) in a Mixture of Formamide with Water at Four Temperatures.Molecules (Basel, Switzerland) · 2023Article
- Excessive addition split peak formed by the non-templated nucleotide addition property ofFrontiers in bioengineering and biotechnology · 2023Article
- Article
- A Simple Multiplex Reverse Transcription-PCR Method for the Diagnosis of L-A and M Totiviruses in Saccharomyces cerevisiae.Applied and environmental microbiology · 2022Article
- Heterogeneity of Starved Yeast Cells in IFFrontiers in microbiology · 2022Article
- Development, validation, and application of the ribosome separation and reconstitution system for protein translation in vitro.RNA (New York, N.Y.) · 2021Article
- A simple methodology for RNA isolation from bacteria by integration of formamide extraction and chitosan-modified silica purification.Analytical and bioanalytical chemistry · 2021Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Current methods for isolating RNA from budding yeast require lengthy and laborious steps such as freezing and heating with phenol, homogenization with glass beads, or enzymatic digestion of the cell wall. Here, extraction with a solution of formamide and EDTA was adapted to isolate RNA from whole yeast cells through a rapid and easily scalable procedure that does not require mechanical cell lysis, phenol, or enzymes. RNA extracted with formamide-EDTA can be directly loaded on gels for electrophoretic analysis without alcohol precipitation. A simplified protocol for downstream DNase treatment and reverse transcription reaction is also included. The formamide-EDTA extraction of yeast RNA is faster, safer, and more economical than conventional methods, outperforms them in terms of total yield, and greatly increases throughput.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.