Evidence map›Paper›PMID 28692343›Full record

ArticleFoodborne pathogens and disease2017

Pulsotype Diversity of Clostridium botulinum Strains Containing Serotypes A and/or B Genes.

Jessica L Halpin, Lavin Joseph, Janet K Dykes, Loretta McCroskey, Elise Smith, Denise Toney, Steven Stroika, Kelley Hise, Susan Maslanka, Carolina Lúquez

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Foodborne pathogens and disease, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.5field-weighted citation impact, top 32% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 5 citations in OpenAlex.

  1. Review
  2. Article
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 2 institutions in 1 country.

Jessica L Halpin1 National Botulism and Enteric Toxins Team, Enteric Diseases Laboratory Branch, Centers for Disease Control and Prevention , Atlanta, Georgia .
Lavin Joseph1 National Botulism and Enteric Toxins Team, Enteric Diseases Laboratory Branch, Centers for Disease Control and Prevention , Atlanta, Georgia .
Janet K Dykes1 National Botulism and Enteric Toxins Team, Enteric Diseases Laboratory Branch, Centers for Disease Control and Prevention , Atlanta, Georgia .
Loretta McCroskey1 National Botulism and Enteric Toxins Team, Enteric Diseases Laboratory Branch, Centers for Disease Control and Prevention , Atlanta, Georgia .
Elise Smith2 PFGE Molecular Subtyping Laboratory, Virginia Division of Consolidated Laboratory Services , Richmond, Virginia.
Denise Toney2 PFGE Molecular Subtyping Laboratory, Virginia Division of Consolidated Laboratory Services , Richmond, Virginia.
Steven Stroika1 National Botulism and Enteric Toxins Team, Enteric Diseases Laboratory Branch, Centers for Disease Control and Prevention , Atlanta, Georgia .
Kelley Hise1 National Botulism and Enteric Toxins Team, Enteric Diseases Laboratory Branch, Centers for Disease Control and Prevention , Atlanta, Georgia .
Susan Maslanka1 National Botulism and Enteric Toxins Team, Enteric Diseases Laboratory Branch, Centers for Disease Control and Prevention , Atlanta, Georgia .
Carolina Lúquez1 National Botulism and Enteric Toxins Team, Enteric Diseases Laboratory Branch, Centers for Disease Control and Prevention , Atlanta, Georgia .
Centers for Disease Control and Prevention · USDepartment of General Services · US

Funding

Intramural CDC HHS CC999999
6 · The paper itself

Abstract

Clostridium botulinum strains are prevalent in the environment and produce a potent neurotoxin that causes botulism, a rare but serious paralytic disease. In 2010, a national PulseNet database was established to curate C. botulinum pulsotypes and facilitate epidemiological investigations, particularly for serotypes A and B strains frequently associated with botulism cases in the United States. Between 2010 and 2014 we performed pulsed-field gel electrophoresis (PFGE) using a PulseNet protocol, uploaded the resulting PFGE patterns into a national database, and analyzed data according to PulseNet criteria (UPGMA clustering, Dice coefficient, 1.5% position tolerance, and 1.5% optimization). A retrospective data analysis was undertaken on 349 entries comprised of type A and B strains isolated from foodborne and infant cases to determine epidemiological relevance, resolution of the method, and the diversity of the database. Most studies to date on the pulsotype diversity of C. botulinum have encompassed very small sets of isolates; this study, with over 300 isolates, is more comprehensive than any published to date. Epidemiologically linked isolates had indistinguishable patterns, except in four instances and there were no obvious geographic trends noted. Simpson's Index of Diversity (D) has historically been used to demonstrate species diversity and abundance within a group, and is considered a standard descriptor for PFGE databases. Simpson's Index was calculated for each restriction endonuclease (SmaI, XhoI), the pattern combination SmaI-XhoI, as well as for each toxin serotype. The D values indicate that both enzymes provided better resolution for serotype B isolates than serotype A. XhoI as the secondary enzyme provided little additional discrimination for C. botulinum. SmaI patterns can be used to exclude unrelated isolates during a foodborne outbreak, but pulsotypes should always be considered concurrently with available epidemiological data.

Indexed as

Bacterial Typing TechniquesBiodiversityBotulismClostridium botulinumElectrophoresis, Gel, Pulsed-FieldEpidemiological MonitoringFoodborne DiseasesHumansInfantRetrospective StudiesSerogroupUnited StatesbotulismClostridium botulinumPFGEsubtypingsurveillance

Identifiers

PMID28692343
PMCPMC5591077
OpenAlexW2735328535

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.