ReviewFrontiers in microbiology2017
Illuminating Messengers: An Update and Outlook on RNA Visualization in Bacteria.
Review in Frontiers in microbiology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
11 citing papers in PubMed, 22 citations in OpenAlex.
- Opticool: Cutting-edge transgenic optical tools.PLoS genetics · 2024Review
- MinD-RNase E interplay controls localization of polar mRNAs in E. coli.The EMBO journal · 2024Article
- Recent advances in tools to map the microbiome.Current opinion in biomedical engineering · 2021Article
- Intracellular mRNA transport and localized translation.Nature reviews. Molecular cell biology · 2021Review
- Rational Design of Aptamer-Tagged tRNAs.International journal of molecular sciences · 2020Article
- A How-To Guide for Mode of Action Analysis of Antimicrobial Peptides.Frontiers in cellular and infection microbiology · 2020Review
- Fluorescence in situ hybridization (FISH) and cell sorting of living bacteria.Scientific reports · 2019Article
- ColiCoords: A Python package for the analysis of bacterial fluorescence microscopy data.PloS one · 2019Article
- RNA Localization in Bacteria.Microbiology spectrum · 2018Review
- Light-Up RNA Aptamers and Their Cognate Fluorogens: From Their Development to Their Applications.International journal of molecular sciences · 2017Review
- Method for Labeling Transcripts in Individual Escherichia coli Cells for Single-molecule Fluorescence In Situ Hybridization Experiments.Journal of visualized experiments : JoVE · 2017Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
To be able to visualize the abundance and spatiotemporal features of RNAs in bacterial cells would permit obtaining a pivotal understanding of many mechanisms underlying bacterial cell biology. The first methods that allowed observing single mRNA molecules in individual cells were introduced by Bertrand et al. (1998) and Femino et al. (1998). Since then, a plethora of techniques to image RNA molecules with the aid of fluorescence microscopy has emerged. Many of these approaches are useful for the large eukaryotic cells but their adaptation to study RNA, specifically mRNA molecules, in bacterial cells progressed relatively slow. Here, an overview will be given of fluorescent techniques that can be used to reveal specific RNA molecules inside fixed and living single bacterial cells. It includes a critical evaluation of their caveats as well as potential solutions.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.