ArticleCurrent biology : CB2017
Maintenance of Miranda Localization in Drosophila Neuroblasts Involves Interaction with the Cognate mRNA.
Article in Current biology : CB, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
14 citing papers in PubMed, 27 citations in OpenAlex.
- Lgl antagonizes Par complex membrane association to enable neural stem cell asymmetric division.Journal of cell science · 2026Article
- A CDK1 phosphorylation site oneLife · 2024Article
- Live-Cell Imaging of Drosophila melanogaster Third Instar Larval Brains.Journal of visualized experiments : JoVE · 2023Article
- Consumption of a polarized membrane reservoir drives asymmetric membrane expansion during the unequal divisions of neural stem cells.Developmental cell · 2023Article
- Pericentrin interacts with Kinesin-1 to drive centriole motility.The Journal of cell biology · 2022Article
- Phase Separation and Mechanical Forces in Regulating Asymmetric Cell Division of Neural Stem Cells.International journal of molecular sciences · 2021Review
- Exploring cellular biochemistry with nanobodies.The Journal of biological chemistry · 2020Review
- Principles and mechanisms of asymmetric cell division.Development (Cambridge, England) · 2020Review
- Asymmetric distribution of glucose transporter mRNA provides a growth advantage in yeast.The EMBO journal · 2019Article
- Using Nanobodies to Study Protein Function in Developing Organisms.Antibodies (Basel, Switzerland) · 2019Review
- A chemical-genetics approach to study the role of atypical Protein Kinase C inDevelopment (Cambridge, England) · 2019Article
- Review
- Emerging mechanisms of asymmetric stem cell division.The Journal of cell biology · 2018Review
- Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
How cells position their proteins is a key problem in cell biology. Targeting mRNAs to distinct regions of the cytoplasm contributes to protein localization by providing local control over translation. Here, we reveal that an interdependence of a protein and cognate mRNA maintains asymmetric protein distribution in mitotic Drosophila neural stem cells. We tagged endogenous mRNA or protein products of the gene miranda that is required for fate determination with GFP. We find that the mRNA localizes like the protein it encodes in a basal crescent in mitosis. We then used GFP-specific nanobodies fused to localization domains to alter the subcellular distribution of the GFP-tagged mRNA or protein. Altering the localization of the mRNA resulted in mislocalization of the protein and vice versa. Protein localization defects caused by mislocalization of the cognate mRNA were rescued by introducing untagged mRNA coding for mutant non-localizable protein. Therefore, by combining the MS2 system and subcellular nanobody expression, we uncovered that maintenance of Mira asymmetric localization requires interaction with the cognate mRNA.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.