ArticleBrazilian journal of microbiology : [publication of the Brazilian Society for Microbiology]
Isolation and characterization of a novel endo-β-1,4-glucanase from a metagenomic library of the black-goat rumen.
Article in Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology]. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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13 citing papers in PubMed, 28 citations in OpenAlex.
- Biochemical and structural characterisation of a family GH5 cellulase from endosymbiont of shipworm P. megotara.Biotechnology for biofuels and bioproducts · 2023Article
- Characterization of Two Novel Rumen-Derived Exo-Polygalacturonases: Catalysis and Molecular Simulations.Microorganisms · 2023Article
- Role of metagenomics in prospecting novel endoglucanases, accentuating functional metagenomics approach in second-generation biofuel production: a review.Biomass conversion and biorefinery · 2023Review
- Heterologous expression and characterization of two novel glucanases derived from sheep rumen microbiota.World journal of microbiology & biotechnology · 2022Article
- A thermo-alkali stable and detergent compatible processive β-1,4-glucanase from HimalayanFrontiers in microbiology · 2022Article
- In silico characterization of the GH5-cellulase family from uncultured microorganisms: physicochemical and structural studies.Journal, genetic engineering & biotechnology · 2021Article
- Heterologous expression and biochemical characterization of a thermostable endo-β-1,4-glucanase from Colletotrichum orchidophilum.Bioprocess and biosystems engineering · 2021Article
- A novel high performance in-silico screened metagenome-derived alkali-thermostable endo-β-1,4-glucanase for lignocellulosic biomass hydrolysis in the harsh conditions.BMC biotechnology · 2020Article
- Characterization of two GH5 endoglucanases from termite microbiome using synthetic metagenomics.Applied microbiology and biotechnology · 2020Article
- Metagenomics as a Tool To Monitor Reclaimed-Water Quality.Applied and environmental microbiology · 2020Review
- A novel bacterial carboxylesterase identified in a metagenome derived-clone from Brazilian mangrove sediments.Molecular biology reports · 2020Article
- Metatranscriptomics of the Hu sheep rumen microbiome reveals novel cellulases.Biotechnology for biofuels · 2019Article
- Isolation and identification of a cellulolyticVeterinary world · 2017Article
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Authors and funding
10 authors at 4 institutions in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
The various types of lignocellulosic biomass found in plants comprise the most abundant renewable bioresources on Earth. In this study, the ruminal microbial ecosystem of black goats was explored because of their strong ability to digest lignocellulosic forage. A metagenomic fosmid library containing 115,200 clones was prepared from the black-goat rumen and screened for a novel cellulolytic enzyme. The KG35 gene, containing a novel glycosyl hydrolase family 5 cellulase domain, was isolated and functionally characterized. The novel glycosyl hydrolase family 5 cellulase gene is composed of a 963-bp open reading frame encoding a protein of 320 amino acid residues (35.1kDa). The deduced amino acid sequence showed the highest sequence identity (58%) for sequences from the glycosyl hydrolase family 5 cellulases. The novel glycosyl hydrolase family 5 cellulase gene was overexpressed in Escherichia coli. Substrate specificity analysis revealed that this recombinant glycosyl hydrolase family 5 cellulase functions as an endo-β-1,4-glucanase. The recombinant KG35 endo-β-1,4-glucanase showed optimal activity within the range of 30-50°C at a pH of 6-7. The thermostability was retained and the pH was stable in the range of 30-50°C at a pH of 5-7.
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