Evidence map›Paper›PMID 28688684›Full record

ArticleOral oncology2017

Early detection of squamous cell carcinoma in carcinogen induced oral cancer rodent model by ratiometric activatable cell penetrating peptides.

Dina V Hingorani, Aaron J Lemieux, Joseph R Acevedo, Heather L Glasgow, Suraj Kedarisetty, Michael A Whitney, Alfredo A Molinolo, Roger Y Tsien, Quyen T Nguyen

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Oral oncology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
1.2field-weighted citation impact, top 21% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 18 citations in OpenAlex.

  1. Article
  2. Review
  3. Article
  4. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 3 institutions in 1 country.

Dina V HingoraniDivision of Otolaryngology, Head and Neck Surgery, University of California, San Diego, La Jolla, CA, USA.
Aaron J LemieuxDivision of Otolaryngology, Head and Neck Surgery, University of California, San Diego, La Jolla, CA, USA.
Joseph R AcevedoDivision of Otolaryngology, Head and Neck Surgery, University of California, San Diego, La Jolla, CA, USA.
Heather L GlasgowDepartment of Microbiology and Immunology, Albert Einstein College of Medicine, New York, NY, USA.
Suraj KedarisettyDivision of Otolaryngology, Head and Neck Surgery, University of California, San Diego, La Jolla, CA, USA.
Michael A WhitneyDepartment of Pharmacology, University of California, San Diego, La Jolla, CA, USA.
Alfredo A MolinoloMoores Cancer Center, University of California San Diego, La Jolla, CA, USA.
Roger Y TsienDepartment of Pharmacology, University of California, San Diego, La Jolla, CA, USA; Moores Cancer Center, University of California San Diego, La Jolla, CA, USA; Howard Hughes Medical Institute, University of California San Diego, La Jolla, CA, USA.
Quyen T NguyenDivision of Otolaryngology, Head and Neck Surgery, University of California, San Diego, La Jolla, CA, USA; Department of Pharmacology, University of California, San Diego, La Jolla, CA, USA; Moores Cancer Center, University of California San Diego, La Jolla, CA, USA. Electronic address: q1nguyen@ucsd.edu.
University of California San Diego · USAlbert Einstein College of Medicine · USHoward Hughes Medical Institute · US

Funding

UC San Diego Clinical and Translational Research InstituteTL1TR001443 · NCATS · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI DEPP, COLIN A. · 2015 to 2019
$3.4M
Injectable reporters to image tumors and guide resectionR01CA158448 · NCI · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI NGUYEN, QUYEN · 2011 to 2015
$3.1M
Testing Fluorescently Labeled Probes for Nerve Imaging during SurgeryR01EB014929 · NIBIB · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI NGUYEN, QUYEN · 2012 to 2016
$1.7M
NCATS NIH HHS TL1 TR001443NCI NIH HHS R01 CA158448NIBIB NIH HHS R01 EB014929
6 · The paper itself

Abstract

objectivesRatiometric cell-penetrating-peptides (RACPP) are hairpin-shaped molecules that undergo cleavage by tumor-associated proteases resulting in measurable Cy5:Cy7 fluorescence ratiometric change to label cancer in vivo. We evaluated an MMP cleavable RACPP for use in the early detection of malignant lesions in a carcinogen-induced rodent tumor model.

methodsWild-type immune-competent mice were given 4-nitroquinoline-oxide (4NQO) for 16weeks. Oral cavities from live mice that had been intravenously administered MMP cleavable PLGC(Me)AG-RACPP were serially imaged from week 11 through week 21 using white-light reflectance and Cy5:Cy7 ratiometric fluorescence.

resultsIn an initial study we found that at week 21 nearly all mice (13/14) had oral cavity lesions, of which 90% were high-grade dysplasia or invasive carcinoma. These high-grade lesions were identifiable with white light reflectance and RACPP Cy5:Cy7 ratiometric fluorescence with similar detectability, Area Under Curve (AUC) for RACPP detection was 0.97 (95% Confidence interval (CI)=0.92-1.02, p<0.001), sensitivity=89%, specificity=100%. In a follow up study, oral cavity lesions generated by 4NQO were imaged and histologically analyzed at weeks 16, 18 and 21. In this study we showed that RACPP-fluorescence detection positively identified 15 squamous cell carcinomas (in 6 separate mice) that were poorly visible or undetectable by white light reflectance.

conclusionsRACPP ratiometric fluorescence can be used to accurately detect carcinogen-induced carcinoma in immunocompetent mice that are poorly visible or undetectable by white light reflectance.

Indexed as

Disease Models, Animal4-Nitroquinoline-1-oxideAnimalsCarcinogensCarcinoma, Squamous CellCell-Penetrating PeptidesFemaleFluorescenceMiceMice, Inbred C57BLMouth NeoplasmsSensitivity and Specificity4-Nitroquinoline-1-oxideCarcinogensCell-Penetrating PeptidesCarcinogen-induced head and neck squamous cell carcinomaEnzyme responsiveIn vivoMatrix metalloproteinaseRatiometricfluorescence imaging

Identifiers

PMID28688684
PMCPMC5575898
OpenAlexW2671226239

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.