ArticleScientific reports2017
Biochemical properties of GH94 cellodextrin phosphorylase THA_1941 from a thermophilic eubacterium Thermosipho africanus TCF52B with cellobiose phosphorylase activity.
Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed.
- Cellulose-functionalized magnetic nanoparticle-mediated isolation of novel cellulolytic bacteria.Applied and environmental microbiology · 2025Article
- Production of cello-oligosaccharides from corncob residue by degradation-synthesis reactions.Applied microbiology and biotechnology · 2024Article
- Glycoside Phosphorylase Catalyzed Cellulose and β-1,3-Glucan Synthesis Using Chromophoric Glycosyl Acceptors.Biomacromolecules · 2024Article
- Broadening the Substrate Specificity of Cellobiose Phosphorylase fromInternational journal of molecular sciences · 2023Article
- Utilization of dietary mixed-linkage β-glucans by the Firmicute Blautia producta.The Journal of biological chemistry · 2023Article
- Insights to improve the activity of glycosyl phosphorylases from RuminococcusFrontiers in chemistry · 2023Article
- Insight into CAZymes ofInternational journal of molecular sciences · 2022Article
- Whole genome sequencing of the halophilic Halomonas qaidamensis XH36, a novel species strain with high ectoine production.Antonie van Leeuwenhoek · 2022Article
- Molecular Recognition of Natural and Non-Natural Substrates by Cellodextrin Phosphorylase from Ruminiclostridium Thermocellum Investigated by NMR Spectroscopy.Chemistry (Weinheim an der Bergstrasse, Germany) · 2021Article
- An Engineered Thermostable Laccase with Great Ability to Decolorize and Detoxify Malachite Green.International journal of molecular sciences · 2021Article
- Kinetic modeling of phosphorylase-catalyzed iterative β-1,4-glycosylation for degree of polymerization-controlled synthesis of soluble cello-oligosaccharides.Biotechnology for biofuels · 2021Article
- β-Glucan phosphorylases in carbohydrate synthesis.Applied microbiology and biotechnology · 2021Review
- Product solubility control in cellooligosaccharide production by coupled cellobiose and cellodextrin phosphorylase.Biotechnology and bioengineering · 2019Article
- Unraveling the subtleties of β-(1→3)-glucan phosphorylase specificity in the GH94, GH149, and GH161 glycoside hydrolase families.The Journal of biological chemistry · 2019Article
- Acceleration of cellodextrin phosphorolysis for bioelectricity generation from cellulosic biomass by integrating a synthetic two-enzyme complex into an in vitro synthetic enzymatic biosystem.Biotechnology for biofuels · 2019Article
- In vitro and in vivo exploration of the cellobiose and cellodextrin phosphorylases panel inBiotechnology for biofuels · 2019Article
- Identification ofThe Journal of biological chemistry · 2018Article
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A hypothetic gene (THA_1941) encoding a putative cellobiose phosphorylase (CBP) from Thermosipho africanus TCF52B has very low amino acid identities (less than 12%) to all known GH94 enzymes. This gene was cloned and over-expressed in Escherichia coli BL21(DE3). The recombinant protein was hypothesized to be a CBP enzyme and it showed an optimum temperature of 75 °C and an optimum pH of 7.5. Beyond its CBP activity, this enzyme can use cellobiose and long-chain cellodextrins with a degree of polymerization of greater than two as a glucose acceptor, releasing phosphate from glucose 1-phosphate. The catalytic efficiencies (k
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