ArticleMolecular & cellular proteomics : MCP2017
Spatiotemporal Proteomic Profiling of Human Cerebral Development.
Article in Molecular & cellular proteomics : MCP, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed, 47 citations in OpenAlex.
- Proteomic analyses in early brain development and neuropathological implications of fetal growth restriction.Neural regeneration research · 2026Article
- A posttranslational proteomic survey of a single anatomically preserved human 20-week postconception brain.Journal of anatomy · 2026Article
- Proteomic profiling reveals dynamic regulation of vesicle trafficking across glioma grades.Journal of neuro-oncology · 2025Article
- Guided Differentiation of Pluripotent Stem Cells into Heterogeneously Differentiating Cultures of Cardiac Cells.bioRxiv : the preprint server for biology · 2025Article
- Human neural rosettes secrete bioactive extracellular vesicles enriched in neuronal and glial cellular components.Scientific reports · 2025Article
- Spatiotemporal multi-omics: exploring molecular landscapes in aging and regenerative medicine.Military Medical Research · 2024Review
- Proteomics applications in next generation induced pluripotent stem cell models.Expert review of proteomics · 2024Review
- Buffering of transcription rate by mRNA half-life is a conserved feature of Rett syndrome models.Nature communications · 2023Article
- Article
- Moving CNS axon growth and regeneration research into human model systems.Frontiers in neuroscience · 2023Review
- Generation of cell-type-specific proteomes of neurodevelopment from human cerebral organoids.STAR protocols · 2022Article
- Conserved exchange of paralog proteins during neuronal differentiation.Life science alliance · 2022Article
- The promise of organoids for unraveling the proteomic landscape of the developing human brain.Molecular psychiatry · 2022Review
- Brain and testis: more alike than previously thought?Open biology · 2021Review
- Quantification of mRNA ribosomal engagement in human neurons using parallel translating ribosome affinity purification (TRAP) and RNA sequencing.STAR protocols · 2021Article
- Protein Interaction Network Biology in Neuroscience.Proteomics · 2021Review
- The quest of cell surface markers for stem cell therapy.Cellular and molecular life sciences : CMLS · 2021Review
- Can gliomas provide insights into promoting synaptogenesis?Molecular psychiatry · 2020Review
- Deciphering the Proteome Dynamics during Development of Neurons Derived from Induced Pluripotent Stem Cells.Journal of proteome research · 2020Article
- Modeling neuronal consequences of autism-associated gene regulatory variants with human induced pluripotent stem cells.Molecular autism · 2020Review
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Mass spectrometry (MS) analysis of human post-mortem central nervous system (CNS) tissue and induced pluripotent stem cell (iPSC)-based directed differentiations offer complementary avenues to define protein signatures of neurodevelopment. Methodological improvements of formalin-fixed, paraffin-embedded (FFPE) protein isolation now enable widespread proteomic analysis of well-annotated archival tissue samples in the context of development and disease. Here, we utilize a shotgun label-free quantification (LFQ) MS method to profile magnetically enriched human cortical neurons and neural progenitor cells (NPCs) derived from iPSCs. We use these signatures to help define spatiotemporal protein dynamics of developing human FFPE cerebral regions. We show that the use of high resolution Q Exactive mass spectrometers now allow simultaneous quantification of >2700 proteins in a single LFQ experiment and provide sufficient coverage to define novel biomarkers and signatures of NPC maintenance and differentiation. Importantly, we show that this abbreviated strategy allows efficient recovery of novel cytoplasmic, membrane-specific and synaptic proteins that are shared between both
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.