Evidence map›Paper›PMID 28686280›Full record

ArticleLab on a chip2017

Spectral reading of optical resonance-encoded cells in microfluidics.

Matjaž Humar, Avinash Upadhya, Seok Hyun Yun

Abstract read
In one paragraph

Article in Lab on a chip, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.

0numbers the graph read from it
0cells of the map it votes in
11citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

11 citing papers in PubMed.

  1. Article
  2. Review
  3. Article
  4. Quantifying local stiffness and forces in soft biological tissues using droplet optical microcavities.Proceedings of the National Academy of Sciences of the United States of America · 2024
    Article
  5. Review
  6. Article
  7. Review
  8. Article
  9. Article
  10. Article
  11. Non-obstructive intracellular nanolasers.Nature communications · 2018
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Matjaž HumarWellman Center for Photomedicine, Harvard Medical School, Massachusetts General Hospital, 65 Landsdowne St. UP-5, Cambridge, Massachusetts 02139, USA. syun@hms.harvard.edu.
Avinash Upadhya
Seok Hyun Yun

Funding

TRD3: Percutaneous and Interstitial ImagingP41EB015903 · NIBIB · MASSACHUSETTS GENERAL HOSPITAL · PI Brett E Bouma · 2012 to 2026
$22.4M
Physics of collective cellular migration in lung health and diseaseP01HL120839 · NHLBI · HARVARD SCHOOL OF PUBLIC HEALTH · PI FREDBERG, JEFFREY J · 2014 to 2018
$12.2M
Massive wavelength-division multiplexing and imaging with laser particlesDP1EB024242 · NIBIB · MASSACHUSETTS GENERAL HOSPITAL · PI YUN, SEOK-HYUN ANDY · 2016 to 2020
$5.8M
Bioluminecence-activated photodynamic therapy of breast cancerR01CA192878 · NCI · MASSACHUSETTS GENERAL HOSPITAL · PI YUN, SEOK-HYUN ANDY · 2015 to 2019
$2.3M
NCI NIH HHS R01 CA192878NHLBI NIH HHS P01 HL120839NIBIB NIH HHS DP1 EB024242NIBIB NIH HHS P41 EB015903
6 · The paper itself

Abstract

The ability to label individual cells is useful for single-cell-level studies of complex cellular interactions and heterogeneity. Optically readable cell labeling is attractive as it can be investigated non-invasively and repeatedly at high speeds. Here, we demonstrate the feasibility of large-scale cell barcoding and identification using fluorescent polystyrene microbeads loaded into cells. Intracellular beads with different diameters in a range of 5 to 12 μm generate spectrally distinguished features or barcodes. A microfluidic chip was used to measure fluorescence resonance peaks emitted from individual cells. An algorithm comparing the peak wavelengths to a reference barcode library allowed barcode identification with high accuracy. This work provides a guideline to increase the number of unique identifiers and reduce various false-positive and false-negative errors.

Indexed as

Electronic Data ProcessingAlgorithmsFluorescent DyesMicrofluidic Analytical TechniquesMicroscopy, FluorescenceMicrospheresPolystyrenesSingle-Cell AnalysisFluorescent DyesPolystyrenes

Identifiers

PMID28686280
PMCPMC5555601

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.