ArticleInternational journal of molecular sciences2017
Transgenic Strategies for Sparse but Strong Expression of Genetically Encoded Voltage and Calcium Indicators.
Article in International journal of molecular sciences, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed.
- Continuous flash suppression responses in mouse visual cortex: Stimulus laterality and anesthesia effects.iScience · 2026Article
- Multimodal optical imaging combining voltage-sensitive dye ElectroFluor630 with genetically encoded calcium, glutamate, or voltage indicators.Neurophotonics · 2026Article
- Noise Sources and Strategies for Signal Quality Improvement in Biological Imaging: A Review Focused on Calcium and Cell Membrane Voltage Imaging.Biosensors · 2026Review
- Cortical Correlates of Psychedelic-Induced Shaking Behavior Revealed by Voltage Imaging.International journal of molecular sciences · 2023Article
- Review
- CaScience advances · 2022Article
- Article
- Population imaging discrepancies between a genetically-encoded calcium indicator (GECI) versus a genetically-encoded voltage indicator (GEVI).Scientific reports · 2021Article
- Studying Synaptically Evoked Cortical ResponsesFrontiers in neuroscience · 2021Article
- Biophysical Parameters of GEVIs: Considerations for Imaging Voltage.Biophysical journal · 2020Review
- Subcellular resolution three-dimensional light-field imaging with genetically encoded voltage indicators.Neurophotonics · 2020Article
- Genetically Encoded Tools for Research of Cell Signaling and Metabolism under Brain Hypoxia.Antioxidants (Basel, Switzerland) · 2020Review
- High-Accuracy Detection of Neuronal Ensemble Activity in Two-Photon Functional Microscopy Using Smart Line Scanning.Cell reports · 2020Article
- Optical voltage imaging in neurons: moving from technology development to practical tool.Nature reviews. Neuroscience · 2019Review
- Engineering Photoactivatability in Genetically Encoded Voltage and pH Indicators.Frontiers in cellular neuroscience · 2019Article
- Single-Neuron Level One-Photon Voltage Imaging With Sparsely Targeted Genetically Encoded Voltage Indicators.Frontiers in cellular neuroscience · 2019Article
- Editorial: New Insights on Neuron and Astrocyte Function From Cutting-Edge Optical Techniques.Frontiers in cellular neuroscience · 2019Article
- Article
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Authors and funding
4 authors.
Funding
Abstract
Rapidly progressing development of optogenetic tools, particularly genetically encoded optical indicators, enables monitoring activities of neuronal circuits of identified cell populations in longitudinal in vivo studies. Recently developed advanced transgenic approaches achieve high levels of indicator expression. However, targeting non-sparse cell populations leads to dense expression patterns such that optical signals from neuronal processes cannot be allocated to individual neurons. This issue is particularly pertinent for the use of genetically encoded voltage indicators whose membrane-delimited signals arise largely from the neuropil where dendritic and axonal membranes of many cells intermingle. Here we address this need for sparse but strong expression of genetically encoded optical indicators using a titratable recombination-activated transgene transcription to achieve a Golgi staining-type indicator expression pattern in vivo. Using different transgenic strategies, we also illustrate that co-expression of genetically encoded voltage and calcium indicators can be achieved in vivo for studying neuronal circuit input-output relationships.
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Registered trials
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