ArticleNature communications2017
Measurement of nanoscale three-dimensional diffusion in the interior of living cells by STED-FCS.
Article in Nature communications, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
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Who cites it
37 citing papers in PubMed.
- Enhancing STED microscopy via fluorescence lifetime unmixing and filtering in two-species SPLIT-STED.Methods in microscopy · 2026Article
- Correction of Background in Fluorescence Correlation Spectroscopy for Accurate Determination of Particle Number.Biomolecules · 2025Article
- Measuring PARP1 mobility at DNA damage sites by segmented fluorescence correlation spectroscopy.Biophysical journal · 2025Article
- Disentangling Timescales of Molecular Kinetics with spFRET using ALEX-FCS.Journal of fluorescence · 2025Article
- Exploiting the detector distance information in image scanning microscopy by phasor-based SPLIT-ISM.Biomedical optics express · 2025Article
- Segmented fluorescence correlation spectroscopy (FCS) on a commercial laser scanning microscope.Scientific reports · 2024Article
- Single-photon microscopy to study biomolecular condensates.Nature communications · 2023Article
- Exploring the Role of the Plant Actin Cytoskeleton: From Signaling to Cellular Functions.International journal of molecular sciences · 2023Review
- SPLIT-PIN software enabling confocal and super-resolution imaging with a virtually closed pinhole.Scientific reports · 2023Article
- Focus image scanning microscopy for sharp and gentle super-resolved microscopy.Nature communications · 2022Article
- Article
- A phasor-based approach to improve optical sectioning in any confocal microscope with a tunable pinhole.Microscopy research and technique · 2022Article
- Diffusion and interaction dynamics of the cytosolic peroxisomal import receptor PEX5.Biophysical reports · 2022Article
- Evaluation of sted super-resolution image quality by image correlation spectroscopy (QuICS).Scientific reports · 2021Article
- Advanced Static and Dynamic Fluorescence Microscopy Techniques to Investigate Drug Delivery Systems.Pharmaceutics · 2021Review
- Fluorescence Fluctuation Spectroscopy enables quantification of potassium channel subunit dynamics and stoichiometry.Scientific reports · 2021Article
- The role of molecular diffusion within dendritic spines in synaptic function.The Journal of general physiology · 2021Review
- Confocal-based fluorescence fluctuation spectroscopy with a SPAD array detector.Light, science & applications · 2021Article
- Nanoscopy for endosomal escape quantification.Nanoscale advances · 2021Review
- Background Reduction in STED-FCS Using a Bivortex Phase Mask.ACS photonics · 2020Article
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Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The observation of molecular diffusion at different spatial scales, and in particular below the optical diffraction limit (<200 nm), can reveal details of the subcellular topology and its functional organization. Stimulated-emission depletion microscopy (STED) has been previously combined with fluorescence correlation spectroscopy (FCS) to investigate nanoscale diffusion (STED-FCS). However, stimulated-emission depletion fluorescence correlation spectroscopy has only been used successfully to reveal functional organization in two-dimensional space, such as the plasma membrane, while, an efficient implementation for measurements in three-dimensional space, such as the cellular interior, is still lacking. Here we integrate the STED-FCS method with two analytical approaches, the recent separation of photons by lifetime tuning and the fluorescence lifetime correlation spectroscopy, to simultaneously probe diffusion in three dimensions at different sub-diffraction scales. We demonstrate that this method efficiently provides measurement of the diffusion of EGFP at spatial scales tunable from the diffraction size down to ∼80 nm in the cytoplasm of living cells.The measurement of molecular diffusion at sub-diffraction scales has been achieved in 2D space using STED-FCS, but an implementation for 3D diffusion is lacking. Here the authors present an analytical approach to probe diffusion in 3D space using STED-FCS and measure the diffusion of EGFP at different spatial scales.
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