ReviewAdvances in protein chemistry and structural biology2017
Sortase Transpeptidases: Structural Biology and Catalytic Mechanism.
Review in Advances in protein chemistry and structural biology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 66 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
66 citing papers in PubMed, 146 citations in OpenAlex.
- Structural and Functional Interrogation of ActiveBiomolecules · 2026Article
- The conserved Pss exopolysaccharide is a common component ofJournal of bacteriology · 2026Article
- Conformational free energy landscape of sorting signal peptides in complex with bacterial sortases.Biophysical journal · 2026Article
- Amino Acid Variants at the P94 Position inBiochemistry · 2026Article
- Article
- Amino acid variants at the P94 position inbioRxiv : the preprint server for biology · 2026Article
- Streptococcus pneumoniae HtrA is a dynamic and monomeric virulence factor capable of forming larger oligomeric complexes.Protein science : a publication of the Protein Society · 2026Article
- A Putative Interaction between the Transmembrane Domains ofThe journal of physical chemistry. B · 2025Article
- inhibitory effect of hyperin on Staphylococcus aureus pathogenicity though interactions with sortase A and sortase B.Scientific reports · 2025Article
- Article
- The Exoproteome and Surfaceome of ToxigenicJournal of proteome research · 2025Article
- Site-Specific and Fluorescently Enhanced Installation of Post-Translational Protein Modifications via Bifunctional Biarsenical Linker.ACS omega · 2024Article
- Biocatalytic Method for Producing an Affinity Resin for the Isolation of Immunoglobulins.Biomolecules · 2024Article
- Phage defence system CBASS is regulated by a prokaryotic E2 enzyme that imitates the ubiquitin pathway.Nature microbiology · 2024Article
- Newly discovered clouting interplay between matrix metalloproteinases structures and novel quaternary Ammonium K21: computational and in-vivo testing.BMC oral health · 2024Article
- Affinity Resins for the Isolation of Immunoglobulins G Obtained Using Biocatalytic Technology.International journal of molecular sciences · 2024Article
- A unique binding mode of P1' Leu-containing target sequences forRSC chemical biology · 2024Article
- Quantifying the Kinetics of Pilus-Specific Sortase-Catalyzed Crosslinking Using High-Performance Liquid Chromatography.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Structural and functional insights of sortases and their interactions with antivirulence compounds.Current research in structural biology · 2024Review
- Self-cyclisation as a general and efficient platform for peptide and protein macrocyclisation.Communications chemistry · 2023Article
6 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
Gram-positive bacteria use sortase cysteine transpeptidase enzymes to covalently attach proteins to their cell wall and to assemble pili. In pathogenic bacteria sortases are potential drug targets, as many of the proteins that they display on the microbial surface play key roles in the infection process. Moreover, the Staphylococcus aureus Sortase A (SaSrtA) enzyme has been developed into a valuable biochemical reagent because of its ability to ligate biomolecules together in vitro via a covalent peptide bond. Here we review what is known about the structures and catalytic mechanism of sortase enzymes. Based on their primary sequences, most sortase homologs can be classified into six distinct subfamilies, called class A-F enzymes. Atomic structures reveal unique, class-specific variations that support alternate substrate specificities, while structures of sortase enzymes bound to sorting signal mimics shed light onto the molecular basis of substrate recognition. The results of computational studies are reviewed that provide insight into how key reaction intermediates are stabilized during catalysis, as well as the mechanism and dynamics of substrate recognition. Lastly, the reported in vitro activities of sortases are compared, revealing that the transpeptidation activity of SaSrtA is at least 20-fold faster than other sortases that have thus far been characterized. Together, the results of the structural, computational, and biochemical studies discussed in this review begin to reveal how sortases decorate the microbial surface with proteins and pili, and may facilitate ongoing efforts to discover therapeutically useful small molecule inhibitors.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.