ArticleMicrobiome2017
Expanding landscapes of the diversified mcr-1-bearing plasmid reservoirs.
Article in Microbiome, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 55 papers, 1 of them a synthesis that pooled it.
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Who cites it
55 citing papers in PubMed, 1 synthesis or guideline pooled it, 106 citations in OpenAlex.
- Fitness costs of mobilised colistin resistance gene 3 (mcr-3): systematic review, epidemiological study, and functional analysis.EBioMedicine · 2025Pooled it
- Emergence of coexistingMicrobiology spectrum · 2026Article
- Egrets Promote the Transmission and Spread of Plasmid-Mediated Colistin Resistance GeneTransboundary and emerging diseases · 2026Article
- A noncanonical intrinsic terminator in the HicAB toxin-antitoxin operon promotes the transmission of conjugative antibiotic resistance plasmids.Nucleic acids research · 2025Article
- A bacterial methyltransferase that initiates biotin synthesis, an attractive anti-ESKAPE druggable pathway.Science advances · 2024Article
- Phenotypic and genetic characterization of antimicrobial resistance in Salmonella enterica serovar Choleraesuis isolates from humans and animals in Spain from 2006 to 2021.The Journal of antimicrobial chemotherapy · 2024Article
- Inter-plasmid transfer of antibiotic resistance genes accelerates antibiotic resistance in bacterial pathogens.The ISME journal · 2024Article
- A new variant of the colistin resistance gene MCR-1 with co-resistance to β-lactam antibiotics reveals a potential novel antimicrobial peptide.PLoS biology · 2023Article
- A Review of Resistance to Polymyxins and Evolving Mobile Colistin Resistance Gene (Antibiotics (Basel, Switzerland) · 2023Review
- Clonal, Plasmidic and Genetic Diversity of Multi-Drug-Resistant Enterobacterales from Hospitalized Patients in Tripoli, Libya.Antibiotics (Basel, Switzerland) · 2023Article
- Prevalence and Genomic Characteristics ofMicrobiology spectrum · 2023Article
- Comparative genomic analysis of Colistin resistant Escherichia coli isolated from pigs, a human and wastewater on colistin withdrawn pig farm.Scientific reports · 2023Article
- Article
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- Transmissibility and Persistence of the Plasmid-Borne Mobile Colistin Resistance Gene,Antibiotics (Basel, Switzerland) · 2022Article
- Escherichia fergusonii, an Underrated Repository for Antimicrobial Resistance in Food Animals.Microbiology spectrum · 2022Article
- Antimicrobial resistance surveillance ofFrontiers in microbiology · 2022Article
- Colistin Resistance and Molecular Characterization of the Genomes ofFrontiers in cellular and infection microbiology · 2022Article
- Molecular Epidemiology and Colistin-Resistant Mechanism ofFrontiers in microbiology · 2022Article
- Convergence of carbapenem resistance and hypervirulence in a highly-transmissible ST11 clone ofVirulence · 2021Article
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Authors and funding
8 authors at 5 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundPolymyxin is a cationic polypeptide antibiotic that can disrupt bacterial cell membrane by interacting with its lipopolysaccharide molecules and is used as a last resort drug against lethal infections by the carbapenem-resistant superbugs (like NDM-1). However, global discovery of the MCR-1 colistin resistance dramatically challenges the newly renewed interest in colistin for clinical use.
methodsThe mcr-1-harboring plasmids were acquired from swine and human Escherichia coli isolated in China, from 2015 to 2016, and subjected to Illumina PacBio RSII and Hi-Seq2000 for full genome sequencing. PCR was applied to close the gap of the assembled contigs. Ori-Finder was employed to predict the replication origin (oriC) in plasmids. The phenotype of MCR-1-producing isolates was evaluated on the LBA plates with various level of colistin. Genetic deletion was used to test the requirement of the initial "ATG" codon for the MCR-1 function.
resultsHere, we report full genomes of over 10 mcr-1-harboring plasmids with diversified replication incompatibilities. A novel hybrid IncI2/IncFIB plasmid pGD17-2 was discovered and characterized from a swine isolate with colistin resistance. Intriguingly, co-occurrence of two unique mcr-1-bearing plasmids (pGD65-3, IncI2, and pGD65-5, IncX4) was detected in a single isolate GD65, which might accelerate dissemination of the mcr-1 under environmental selection pressure. Genetic analyses of these plasmids mapped mobile elements in the context of antibiotic resistance and determined two insertion sequences (ISEcp1 and ISApl1) that are responsible for the mobilization of mcr-1. Gene deletion also proved that the first ATG codon is redundant in the mcr-1 gene.
conclusionsCollectively, our results extend landscapes of the diversified mcr-1-bearing plasmid reservoirs.
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