Evidence map›Paper›PMID 28683186›Full record

ArticleMacromolecular bioscience2017

Enzyme-Induced Matrix Softening Regulates Hepatocarcinoma Cancer Cell Phenotypes.

Youyun Liang, Nicholas Edwin Clay, Kathryn M Sullivan, Jiayu Leong, Altug Ozcelikkale, Max H Rich, Min Kyung Lee, Mei-Hsiu Lai, Hojeong Jeon, Bumsoo Han and 2 more

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Macromolecular bioscience, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
0.4field-weighted citation impact, top 43% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed, 16 citations in OpenAlex.

  1. Review
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  5. Article
  6. Proteinaceous Hydrogels for Bioengineering Advanced 3D Tumor Models.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2021
    Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors at 4 institutions in 3 countries.

Youyun LiangDepartment of Chemical and Biomolecular Engineering, National University of Singapore, Singapore, 117576, Singapore.
Nicholas Edwin ClayDepartment of Chemical and Biomolecular Engineering, University of Illinois at Urbana-Champaign, Urbana, IL, 61801, USA.ORCID 0000-0002-0343-8812
Kathryn M SullivanDepartment of Chemical and Biomolecular Engineering, University of Illinois at Urbana-Champaign, Urbana, IL, 61801, USA.
Jiayu LeongDepartment of Chemical and Biomolecular Engineering, University of Illinois at Urbana-Champaign, Urbana, IL, 61801, USA.
Altug OzcelikkaleSchool of Mechanical Engineering, Birck Nanotechnology Center, Purdue University, West Lafayette, IN, 47907, USA.
Max H RichDepartment of Chemical and Biomolecular Engineering, University of Illinois at Urbana-Champaign, Urbana, IL, 61801, USA.
Min Kyung LeeDepartment of Chemical and Biomolecular Engineering, University of Illinois at Urbana-Champaign, Urbana, IL, 61801, USA.
Mei-Hsiu LaiDepartment of Chemical and Biomolecular Engineering, University of Illinois at Urbana-Champaign, Urbana, IL, 61801, USA.
Hojeong JeonCenter for Biomaterials, Biomedical Research Institute, Korean Institute of Science and Technology, Seoul, 02792, South Korea.
Bumsoo HanSchool of Mechanical Engineering, Birck Nanotechnology Center, Purdue University, West Lafayette, IN, 47907, USA.
Yen Wah TongDepartment of Chemical and Biomolecular Engineering, National University of Singapore, Singapore, 117576, Singapore.
Hyunjoon KongDepartment of Chemical and Biomolecular Engineering, University of Illinois at Urbana-Champaign, Urbana, IL, 61801, USA.
University of Illinois Urbana-Champaign · USPurdue University West Lafayette · USKorean Association Of Science and Technology Studies · KRNational University of Singapore · SG

Funding

Nanocells for vascular normalization therapiesR01HL109192 · NHLBI · UNIVERSITY OF ILLINOIS AT URBANA-CHAMPAIGN · PI KONG, HYUNJOON · 2011 to 2015
$1.9M
Training the Next Generation of Researchers in Cancer Nanotechnology at the NCI MR25CA154015 · NCI · UNIVERSITY OF ILLINOIS AT URBANA-CHAMPAIGN · PI BASHIR, RASHID, NARDULLI, ANN · 2010 to 2014
$1.5M
NCI NIH HHS R25 CA154015NHLBI NIH HHS R01 HL109192
6 · The paper itself

Abstract

The progression of cancer is often accompanied by changes in the mechanical properties of an extracellular matrix. However, limited efforts have been made to reproduce these biological events in vitro. To this end, this study demonstrates that matrix remodeling caused by matrix metalloproteinase (MMP)-1 regulates phenotypic activities and modulates radiosensitivity of cancer cells exclusively in a 3D matrix. In this study, hepatocarcinoma cells are cultured in a collagen-based gel tailored to present an elastic modulus of ≈4.0 kPa. The subsequent exposure of the gel to MMP-1 decreases the elastic modulus from 4.0 to 0.5 kPa. In response to MMP-1, liver cancer cells undergo active proliferation, downregulation of E-cadherin, and the loss of detoxification capacity. The resulting spheroids are more sensitive to radiation than the spheroids cultured in the stiffer gel not exposed to MMP-1. Overall, this study serves to better understand and control the effects of MMP-induced matrix remodeling.

Indexed as

Radiation ToleranceAntigens, CDCadherinsCarcinoma, HepatocellularCell ProliferationElastic ModulusExtracellular MatrixGene Expression Regulation, NeoplasticHep G2 CellsHumansLiver NeoplasmsMatrix Metalloproteinase 1Antigens, CDCadherinsCDH1 protein, humanMatrix Metalloproteinase 1MMP1 protein, humancollagendegradationextracellular matrix (ECM)hydrogelsmatrix metalloproteinases

Identifiers

PMID28683186
PMCPMC5784765
OpenAlexW2725192324

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.