ArticlePloS one2017
High-throughput electrophysiological assays for voltage gated ion channels using SyncroPatch 768PE.
Article in PloS one, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed.
- Non-invasive electrical stimulation restores corneal nerve density and sensory function in diabetic neuropathy via KCNN-dependent mechanism.Communications biology · 2026Article
- High-throughput methods for cardiac cellular electrophysiology studies: the road to personalized medicine.American journal of physiology. Heart and circulatory physiology · 2024Review
- Ready-to-Record Cells for Kinetic Screening of VGICs.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Article
- A Comparative Study on the Lysosomal Cation Channel TMEM175 Using Automated Whole-Cell Patch-Clamp, Lysosomal Patch-Clamp, and Solid Supported Membrane-Based Electrophysiology: Functional Characterization and High-Throughput Screening Assay Development.International journal of molecular sciences · 2023Article
- Protein 14-3-3 Influences the Response of the Cardiac Sodium Channel NaThe Journal of pharmacology and experimental therapeutics · 2023Article
- Targeting ion channels with ultra-large library screening for hit discovery.Frontiers in molecular neuroscience · 2023Review
- Article
- A High-Throughput Screening Assay to Identify Drugs that Can Treat Long QT Syndrome Caused by Trafficking-Deficient KMolecular pharmacology · 2022Article
- GFP-Margatoxin, a Genetically Encoded Fluorescent Ligand to Probe Affinity of Kv1.3 Channel Blockers.International journal of molecular sciences · 2022Article
- There is no F in APC: Using physiological fluoride-free solutions for high throughput automated patch clamp experiments.Frontiers in molecular neuroscience · 2022Article
- Highly Parallelized, Multicolor Optogenetic Recordings of Cellular Activity for Therapeutic Discovery Applications in Ion Channels and Disease-Associated Excitable Cells.Frontiers in molecular neuroscience · 2022Article
- Review
- Advancing Ion Channel Research with Automated Patch Clamp (APC) Electrophysiology Platforms.Advances in experimental medicine and biology · 2021Article
- Sensing Senses: Optical Biosensors to Study Gustation.Sensors (Basel, Switzerland) · 2020Review
- Deep Mutational Scan of anCirculation. Genomic and precision medicine · 2020Article
- A nonlinear and time-dependent leak current in the presence of calcium fluoride patch-clamp seal enhancer.Wellcome open research · 2020Article
- Feasibility of Using Adjunctive Optogenetic Technologies in Cardiomyocyte Phenotyping - from the Single Cell to the Whole Heart.Current pharmaceutical biotechnology · 2020Review
- Intracellular Recording of Human Cardiac Action Potentials on Market-Available Multielectrode Array Platforms.Frontiers in bioengineering and biotechnology · 2020Article
- Mitragynine, an euphoric compound inhibits hERG1a/1b channel current and upregulates the complexation of hERG1a-Hsp90 in HEK293-hERG1a/1b cells.Scientific reports · 2019Article
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Ion channels regulate a variety of physiological processes and represent an important class of drug target. Among the many methods of studying ion channel function, patch clamp electrophysiology is considered the gold standard by providing the ultimate precision and flexibility. However, its utility in ion channel drug discovery is impeded by low throughput. Additionally, characterization of endogenous ion channels in primary cells remains technical challenging. In recent years, many automated patch clamp (APC) platforms have been developed to overcome these challenges, albeit with varying throughput, data quality and success rate. In this study, we utilized SyncroPatch 768PE, one of the latest generation APC platforms which conducts parallel recording from two-384 modules with giga-seal data quality, to push these 2 boundaries. By optimizing various cell patching parameters and a two-step voltage protocol, we developed a high throughput APC assay for the voltage-gated sodium channel Nav1.7. By testing a group of Nav1.7 reference compounds' IC50, this assay was proved to be highly consistent with manual patch clamp (R > 0.9). In a pilot screening of 10,000 compounds, the success rate, defined by > 500 MΩ seal resistance and >500 pA peak current, was 79%. The assay was robust with daily throughput ~ 6,000 data points and Z' factor 0.72. Using the same platform, we also successfully recorded endogenous voltage-gated potassium channel Kv1.3 in primary T cells. Together, our data suggest that SyncroPatch 768PE provides a powerful platform for ion channel research and drug discovery.
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