Evidence map›Paper›PMID 28682554›Full record

ArticleHua xi kou qiang yi xue za zhi = Huaxi kouqiang yixue zazhi = West China journal of stomatology2017

[Effects of cytosolic bacteria on cyclic GMP-AMP synthase expression in human gingival tissues and periodontal ligament cells].

Yang Xiaojun, Tan Yongmei, Tian Zhihui, Zhou Ting, Zhao Wanghong, Hou Jin

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Hua xi kou qiang yi xue za zhi = Huaxi kouqiang yixue zazhi = West China journal of stomatology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact, top 77% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 0 citations in OpenAlex.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 1 institution in 1 country.

Yang XiaojunDept. of Stomatology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China;College of Stomatology, Southern Medical University, Guangzhou 510515, China.
Tan YongmeiDept. of Stomatology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China;College of Stomatology, Southern Medical University, Guangzhou 510515, China.
Tian ZhihuiDept. of Stomatology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China;College of Stomatology, Southern Medical University, Guangzhou 510515, China.
Zhou TingDept. of Stomatology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China;College of Stomatology, Southern Medical University, Guangzhou 510515, China.
Zhao WanghongDept. of Stomatology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China;College of Stomatology, Southern Medical University, Guangzhou 510515, China.
Hou JinDept. of Stomatology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China;College of Stomatology, Southern Medical University, Guangzhou 510515, China.
Southern Medical University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

objectiveThis work aims to determine the effect of cytosolic bacteria on the expression of cyclic GMP-AMP synthase (cGAS) in human periodontal ligament cells (hPDLCs) and gingival tissues.

methodsThe ability of Porphyromonas gingivalis (P. gingivalis) to invade hPDLCs was detected using laser scanning confocal microscope assay at a multiplicity of infection of 10. P. gingivalis-infected cells were sorted by fluorescence-activated cell sorting (FACS). Then, quantitative real time reverse transcription polymerase chain reaction (qRT-PCR) and Western blot were used to detect cGAS expression in infected cells. Finally, the location and expression of cGAS in inflammatory and normal gingival tissues were investigated by immunohistochemistry.

resultsP. gingivalis actively invaded hPDLCs. Moreover, cGAS expression significantly increased in P. gingivalis-infected cells. Although cGAS was expressed in the epithelial and subepithelial cells of both inflamed and normal gingival tissues, cGAS expression significantly increased in inflamed gingival tissues.

conclusionsCytosolic bacteria can upregulate cGAS expression in infected cells. These data suggest that cGAS may act as pattern-recognition receptors and participate in recognizing cytosolic nucleic acid pathogen-associated molecular patterns.
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Indexed as

GingivaNucleotides, CyclicPeriodontal LigamentPorphyromonas gingivalisBlotting, WesternCells, CulturedFlow CytometryHumansReal-Time Polymerase Chain Reactioncyclic guanosine monophosphate-adenosine monophosphateNucleotides, Cycliccyclic GMP-AMP synthaseDNA sensorhuman periodontal ligament cellsPorphyromonas gingivalis

Identifiers

PMID28682554
PMCPMC7029996
OpenAlexW2990160401

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.