Evidence map›Paper›PMID 28682553›Full record

ArticleHua xi kou qiang yi xue za zhi = Huaxi kouqiang yixue zazhi = West China journal of stomatology2017

[A preliminary study on the autophagy level of human periodontal ligament cells regulated by nicotine].

Du Yang, Yuan Shuai, Zhou Zhifei, Wu Lizheng, Wang Lulu, Wu Xing'an, Wang Xiaojing

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Hua xi kou qiang yi xue za zhi = Huaxi kouqiang yixue zazhi = West China journal of stomatology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.5field-weighted citation impact, top 30% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 5 citations in OpenAlex.

  1. Review
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 1 institution in 1 country.

Du YangState Key Laboratory of Military Stomatology, National Clinical Research Center for Oral Diseases, Shanxi Clinical Research Center for Oral Diseases, Dept. of Pediatric Dentistry, School of Stomatology, The Fourth Military Medical University, Xi'an 710032, China.
Yuan ShuaiState Key Laboratory of Military Stomatology, National Clinical Research Center for Oral Diseases, Shanxi Clinical Research Center for Oral Diseases, Dept. of Pediatric Dentistry, School of Stomatology, The Fourth Military Medical University, Xi'an 710032, China.
Zhou ZhifeiState Key Laboratory of Military Stomatology, National Clinical Research Center for Oral Diseases, Shanxi Clinical Research Center for Oral Diseases, Dept. of Pediatric Dentistry, School of Stomatology, The Fourth Military Medical University, Xi'an 710032, China.
Wu LizhengDept. of Stomatology, Affiliated Hospital of Logistics University of People's Armed Police Force, Tianjin 300300, China.
Wang LuluState Key Laboratory of Military Stomatology, National Clinical Research Center for Oral Diseases, Shanxi Clinical Research Center for Oral Diseases, Dept. of Pediatric Dentistry, School of Stomatology, The Fourth Military Medical University, Xi'an 710032, China.
Wu Xing'anDept. of Microbiology, The Fourth Military Medical University, Xi'an 710032, China.
Wang XiaojingState Key Laboratory of Military Stomatology, National Clinical Research Center for Oral Diseases, Shanxi Clinical Research Center for Oral Diseases, Dept. of Pediatric Dentistry, School of Stomatology, The Fourth Military Medical University, Xi'an 710032, China.
Air Force Medical University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

objectiveTo explore the effect of nicotine on the autophagy level of human periodontal ligament cells (hPDLCs).

methodsPeriodontal tissues collected from premolars for orthodontic treatment reasons were used to culture hPDLCs. Western blot analysis was performed to test the most optimal time and concentration of nicotine on the autophagy level of the hPDLCs. Transmission electron microscope and immunofluorescence observation were carried out to detect the form of autophagosomes and expression of autophagy related protein LC3 in hPDLCs under this optimal condition.

resultsProtein expression of LC3Ⅱ was up regulated with the 12 h nicotine stimulating. Besides that, the up regulation of the protein expression of LC3Ⅱ was concentration dependent and nicotine with a concentration of 1×10⁻⁵ mol·L⁻¹ was the most optimal condition. Transmission electron microscope and immunofluorescence observations indicated that nicotine would activate the autophagy level of hPDLCs by increasing the number of autophagosomes and up regulating the expression of autophagy related protein LC3.

conclusionsNicotine could increase autophagy level of hPDLCs, thus affecting the occurrence and development of smoking related periodontitis.

Indexed as

AutophagyPeriodontal LigamentPeriodontitisBlotting, WesternCell Culture TechniquesCells, CulturedHumansMicrotubule-Associated ProteinsNicotineNicotinic AgonistsUp-RegulationMAP1LC3A protein, humanMicrotubule-Associated ProteinsNicotineNicotinic Agonistsautophagyhuman periodontal ligament cellsnicotine

Identifiers

PMID28682553
PMCPMC7030003
OpenAlexW2976248073

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.