ArticleJournal of the American Society for Mass Spectrometry2017
Top-Down LESA Mass Spectrometry Protein Analysis of Gram-Positive and Gram-Negative Bacteria.
Article in Journal of the American Society for Mass Spectrometry, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed.
- Surface-sampling mass spectrometry to study proteins and protein complexes.Essays in biochemistry · 2023Review
- Probing Interkingdom Signaling Molecules via Liquid Extraction Surface Analysis-Mass Spectrometry.Analytical chemistry · 2023Article
- Direct Analysis of Bio-Molecules in Solid Materials Using Electrospray Ionisation Mass Spectrometry Coupled with Laser Ablation and a Liquid Sampling Technique.Mass spectrometry (Tokyo, Japan) · 2023Article
- Identifying Clinically Relevant Bacteria Directly from Culture and Clinical Samples with a Handheld Mass Spectrometry Probe.Clinical chemistry · 2022Article
- Native ambient mass spectrometry of intact protein assemblies directly fromChemical communications (Cambridge, England) · 2022Article
- Rapid MRSA detection via tandem mass spectrometry of the intact 80 kDa PBP2a resistance protein.Scientific reports · 2021Article
- Liquid Extraction Surface Analysis Mass Spectrometry of ESKAPE Pathogens.Journal of the American Society for Mass Spectrometry · 2021Article
- Direct Liquid Extraction and Ionization Techniques for Understanding Multimolecular Environments in Biological Systems (Secondary Publication).Mass spectrometry (Tokyo, Japan) · 2021Article
- Top-down proteomics: challenges, innovations, and applications in basic and clinical research.Expert review of proteomics · 2020Article
- Direct identification of bacterial and human proteins from infected wounds in living 3D skin models.Scientific reports · 2020Article
- LESA Cyclic Ion Mobility Mass Spectrometry of Intact Proteins from Thin Tissue Sections.Analytical chemistry · 2020Article
- In situ mass spectrometry analysis of intact proteins and protein complexes from biological substrates.Biochemical Society transactions · 2020Review
- Electroporation and Mass Spectrometry: A New Paradigm for In Situ Analysis of Intact Proteins Direct from Living Yeast Colonies.Analytical chemistry · 2020Article
- Probing the Fundamentals of Native Liquid Extraction Surface Analysis Mass Spectrometry of Proteins: Can Proteins Refold during Extraction?Analytical chemistry · 2019Article
- Utilisation of Ambient Laser Desorption Ionisation Mass Spectrometry (ALDI-MS) Improves Lipid-Based Microbial Species Level Identification.Scientific reports · 2019Article
- Ambient ionisation mass spectrometry for in situ analysis of intact proteins.Journal of mass spectrometry : JMS · 2018Article
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Authors and funding
5 authors.
Funding
Abstract
We have previously shown that liquid extraction surface analysis (LESA) mass spectrometry (MS) is a technique suitable for the top-down analysis of proteins directly from intact colonies of the Gram-negative bacterium Escherichia coli K-12. Here we extend the application of LESA MS to Gram-negative Pseudomonas aeruginosa PS1054 and Gram-positive Staphylococcus aureus MSSA476, as well as two strains of E. coli (K-12 and BL21 mCherry) and an unknown species of Staphylococcus. Moreover, we demonstrate the discrimination between three species of Gram-positive Streptococcus (Streptococcus pneumoniae D39, and the viridans group Streptococcus oralis ATCC 35037 and Streptococcus gordonii ATCC35105), a recognized challenge for matrix-assisted laser desorption ionization time-of-flight MS. A range of the proteins detected were selected for top-down LESA MS/MS. Thirty-nine proteins were identified by top-down LESA MS/MS, including 16 proteins that have not previously been observed by any other technique. The potential of LESA MS for classification and characterization of novel species is illustrated by the de novo sequencing of a new protein from the unknown species of Staphylococcus. Graphical Abstract ᅟ.
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