Evidence map›Paper›PMID 28681159›Full record

ArticlePlant cell reports2017

Selectable marker independent transformation of recalcitrant maize inbred B73 and sorghum P898012 mediated by morphogenic regulators BABY BOOM and WUSCHEL2.

Muruganantham Mookkan, Kimberly Nelson-Vasilchik, Joel Hague, Zhanyuan J Zhang, Albert P Kausch

Open access · hybridAbstract read
In one paragraph

Article in Plant cell reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 82 papers.

0numbers the graph read from it
0cells of the map it votes in
82citing papers in PubMed
24.3field-weighted citation impact, top 1% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

82 citing papers in PubMed, 158 citations in OpenAlex.

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  18. Plant engineering: advances, bottlenecks, and promise.The Plant journal : for cell and molecular biology · 2025
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  20. Review

22 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 3 institutions in 1 country.

Muruganantham MookkanPlant Transformation Core Facility, Division of Plant Sciences, University of Missouri, 1-33 Agriculture Building, Columbia, MO, 65211, USA.
Kimberly Nelson-VasilchikDepartment of Cell and Molecular Biology, University of Rhode Island, 530 Liberty Lane, West Kingston, RI, 02892, USA.
Joel HagueDepartment of Cell and Molecular Biology, University of Rhode Island, 530 Liberty Lane, West Kingston, RI, 02892, USA.
Zhanyuan J ZhangPlant Transformation Core Facility, Division of Plant Sciences, University of Missouri, 1-33 Agriculture Building, Columbia, MO, 65211, USA. zhangzh@missouri.edu.
Albert P KauschDepartment of Cell and Molecular Biology, University of Rhode Island, 530 Liberty Lane, West Kingston, RI, 02892, USA. apkausch@uri.edu.
University of Rhode Island · USPlant (United States) · USUniversity of Missouri · US

Funding

National Science Foundation Plant Genome 1444478
6 · The paper itself

Abstract

key messageDiscriminatory co-expression of maize BBM and WUS transcriptional factor genes promoted somatic embryogenesis and efficient Agrobacterium -mediated transformation of recalcitrant maize inbred B73 and sorghum P898012 genotypes without use of a selectable marker gene. The use of morphogenic regulators to overcome barriers in plant transformation is a revolutionary breakthrough for basic plant science and crop applications. Current standard plant transformation systems are bottlenecks for genetic, genomic, and crop improvement studies. We investigated the differential use of co-expression of maize transcription factors BABY BOOM and WUSCHEL2 coupled with a desiccation inducible CRE/lox excision system to enable regeneration of stable transgenic recalcitrant maize inbred B73 and sorghum P898012 without a chemical selectable marker. The PHP78891 expression cassette contains CRE driven by the drought inducible maize RAB17

Indexed as

Gene Expression Regulation, PlantAgrobacterium tumefaciensDroughtsGenetic MarkersGenotypeGreen Fluorescent ProteinsModels, GeneticPlant ProteinsPlants, Genetically ModifiedPromoter Regions, GeneticSorghumTranscription FactorsTransformation, GeneticZea maysGenetic MarkersGreen Fluorescent ProteinsPlant ProteinsTranscription FactorsBABY BOOMMaizeMorphogenic regulatorsSorghumWUSCHEL2

Identifiers

PMID28681159
PMCPMC5565672
OpenAlexW2732418005

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.