ArticleScientific reports2017
Comparison of traditional culture and molecular qPCR for detection of simultaneous carriage of multiple pneumococcal serotypes in African children.
Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 38 citations in OpenAlex.
- Effect of Biannual Azithromycin to Children under 5 Years on the Carriage of Respiratory Pathogens among Children Aged 7-11 Years.The American journal of tropical medicine and hygiene · 2023Trial
- Persistence of Vaccine Serotype Carriage and Differences in Pneumococcal Carriage by Laboratory Method and Sample Type in Indigenous Individuals in the Southwest United States.The Journal of infectious diseases · 2025Observational
- Detection of Pneumococcal Carriage in Asymptomatic Healthcare Workers.Open forum infectious diseases · 2025Article
- A low-cost culture- and DNA extraction-free method for the molecular detection of pneumococcal carriage in saliva.Microbiology spectrum · 2024Article
- Contact with young children is a major risk factor for pneumococcal colonization in older adults.FEMS microbes · 2024Article
- Optimization of a high-throughput nanofluidic real-time PCR to detect and quantify of 15 bacterial species and 92 Streptococcus pneumoniae serotypes.Scientific reports · 2023Article
- Antibiotic resistance ofFrontiers in public health · 2023Article
- Pneumococcal concentration and serotype distribution in preschool children with radiologically confirmed pneumonia compared to healthy controls prior to introduction of pneumococcal vaccination in Zanzibar: an observational study.BMC infectious diseases · 2022Observational
- High prevalence of multiple serotypes of pneumococci in patients with pneumonia and their associated risk factors.Thorax · 2022Article
- Pneumococcal carriage in unvaccinated children at the time of vaccine implementation into the national immunization program in Poland.Scientific reports · 2022Article
- Approximate likelihood-based estimation method of multiple-type pathogen interactions: An application to longitudinal pneumococcal carriage data.Statistics in medicine · 2022Article
- High-throughput nanofluidic real-time PCR to discriminate Pneumococcal Conjugate Vaccine (PCV)-associated serogroups 6, 18, and 22 to serotypes using modified oligonucleotides.Scientific reports · 2021Article
- The upper respiratory tract microbiome of indigenous Orang Asli in north-eastern Peninsular Malaysia.NPJ biofilms and microbiomes · 2021Article
- Association of Laboratory Methods, Colonization Density, and Age With Detection of Streptococcus pneumoniae in the Nasopharynx.American journal of epidemiology · 2019Article
- Nasopharyngeal carriage of Streptococcus pneumoniae serotypes among children in India prior to the introduction of pneumococcal conjugate vaccines: a cross-sectional study.BMC infectious diseases · 2019Article
- Performance of the Biomark HD real-time qPCR System (Fluidigm) for the detection of nasopharyngeal bacterial pathogens and Streptococcus pneumoniae typing.Scientific reports · 2019Article
- Pneumococcal carriage and serotype distribution among children with and without pneumonia in Mozambique, 2014-2016.PloS one · 2018Article
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Corrections and comments
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Authors and funding
3 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
S. pneumoniae is a common colonizer of the human nasopharynx in high income and low-middle income countries. Due to limitations of standard culture methods, the prevalence of concurrent colonization with multiple serotypes is unclear. We evaluated the use of multiplex quantitative PCR (qPCR) to detect multiple pneumococcal serotypes/group colonization in archived nasopharyngeal swabs of pneumococcal conjugate vaccine naive children who had previously been investigated by traditional culture methods. Overall the detection of pneumococcal colonization was higher by qPCR (82%) compared to standard culture (71%; p < 0.001), with a high concordance (kappa = 0.73) of serotypes/groups identified by culture also being identified by qPCR. Also, qPCR was more sensitive in detecting multiple serotype/groups among colonized cases (28.7%) compared to culture (4.5%; p < 0.001). Of the additional serotypes detected only by qPCR, the majority were of lower density (<10
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.