Evidence map›Paper›PMID 28679425›Full record

ArticleStem cell research & therapy2017

Overexpression of c-Met in bone marrow mesenchymal stem cells improves their effectiveness in homing and repair of acute liver failure.

Kun Wang, Yuwen Li, Tiantian Zhu, Yongting Zhang, Wenting Li, Wenyu Lin, Jun Li, Chuanlong Zhu

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Stem cell research & therapy, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
34citing papers in PubMed, 1 pooled it
2.2field-weighted citation impact, top 12% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

34 citing papers in PubMed, 1 synthesis or guideline pooled it, 53 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 3 institutions in 2 countries.

Kun WangDepartment of Infectious Disease, the First Affiliated Hospital with Nanjing Medical University, Nanjing, China.
Yuwen LiDepartment of Pediatrics, the First Affiliated Hospital with Nanjing Medical University, Nanjing, China.
Tiantian ZhuDepartment of Infectious Disease, the First Affiliated Hospital with Nanjing Medical University, Nanjing, China.
Yongting ZhangDepartment of Infectious Disease, the First Affiliated Hospital with Nanjing Medical University, Nanjing, China.
Wenting LiDepartment of Infectious Disease, Anhui Provincial Hospital, Anhui Medical University, Hefei, China.
Wenyu LinLiver Center and Gastrointestinal Division, Department of Medicine, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts, USA.
Jun LiDepartment of Infectious Disease, the First Affiliated Hospital with Nanjing Medical University, Nanjing, China.
Chuanlong ZhuDepartment of Infectious Disease, the First Affiliated Hospital with Nanjing Medical University, Nanjing, China. chuanlong@yahoo.com.ORCID 0000-0002-0657-828X
Nanjing Medical University · CNAnhui Medical University · CNHarvard University · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundTransplantation of bone marrow-derived mesenchymal stem cells (BMSCs) has emerged as a novel therapy for acute liver failure (ALF). However, the homing efficiency of BMSCs to the injured liver sites appears to be poor. In this study, we aimed to determine if overexpression of c-Met in BMSCs could promote the homing ability of BMSCs to rat livers affected by ALF.

methodsOverexpression of c-Met in BMSCs (c-Met-BMSCs) was attained by transfection of naive BMSCs with the lenti-c-Met-GFP. The impact of transplanted c-Met-BMSCs on both homing and repair of ALF was evaluated and compared with lenti-GFP empty vector transfected BMSCs (control BMSCs).

resultsAfter cells were transfected with the lenti-c-Met-GFP vector, the BMSCs displayed very high expression of c-Met protein as demonstrated by Western blot. In addition, in vitro transwell migration assays showed that the migration ability of c-Met-BMSCs was significantly increased in comparison with that of control BMSCs (P < 0.05), and was dependent on hepatocyte growth factor (HGF). Furthermore, rats with ALF that received transplanted c-Met-BMSCs showed significantly improved homing ability to the injured liver; this was accompanied by elevated survival rates and liver function in the ALF rats. Parallel pathological examination further confirmed that transplantation of c-Met-BMSCs ameliorated liver injury with reduced hepatic activity index (HAI) scores, and that the effects of c-Met-BMSCs were more profound than those of control BMSCs.

conclusionsOverexpression of c-Met promotes the homing of BMSCs to injured hepatic sites in a rat model of ALF, thereby improving the efficacy of BMSC therapy for ALF repair.

Indexed as

LiverLiver Failure, AcuteMesenchymal Stem Cell TransplantationAnimalsBone Marrow CellsDisease Models, AnimalLentivirusMaleMesenchymal Stem CellsProto-Oncogene Proteins c-metRatsRats, Sprague-DawleyTransduction, GeneticProto-Oncogene Proteins c-metAcute liver failureBone marrow mesenchymal stem cellsc-MetLentiviral vectorLiver injury

Identifiers

PMID28679425
PMCPMC5499016
OpenAlexW2730810791

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.