ArticleMicrobial cell factories2017
Development of a counterselectable seamless mutagenesis system in lactic acid bacteria.
Article in Microbial cell factories, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
22 citing papers in PubMed, 36 citations in OpenAlex.
- Genetically Modified Lactic Acid Bacteria in the EU Food Chain: Applications, Benefits, and Risk Assessment.International journal of molecular sciences · 2026Review
- Development of a counterselection system for efficient marker-free genetic manipulation inApplied and environmental microbiology · 2026Article
- Genome-Editing Tools for Lactic Acid Bacteria: Past Achievements, Current Platforms, and Future Directions.International journal of molecular sciences · 2025Review
- Pioneering precision in markerless strain development for Synechococcus sp. PCC 7002.Microbial cell factories · 2024Article
- Review
- A convenient broad-host counterselectable system endowing rapid genetic manipulations of Kluyveromyces lactis and other yeast species.Microbial cell factories · 2024Article
- Efficient markerless genetic manipulation ofApplied and environmental microbiology · 2024Article
- Development of a counterselectable system for rapid and efficient CRISPR-based genome engineering in Zymomonas mobilis.Microbial cell factories · 2023Article
- Advances in Genetic Tools and Their Application inFoods (Basel, Switzerland) · 2023Review
- Synthetic biology tools for engineeringComputational and structural biotechnology journal · 2023Review
- New Effective Method of Lactococcus Genome Editing Using Guide RNA-Directed Transposition.International journal of molecular sciences · 2022Article
- Genetic tools for the development of recombinant lactic acid bacteria.Microbial cell factories · 2021Review
- CRISPR-Cas-mediated gene editing in lactic acid bacteria.Molecular biology reports · 2020Review
- The Microbiome as a Therapeutic Target for Multiple Sclerosis: Can Genetically Engineered Probiotics Treat the Disease?Diseases (Basel, Switzerland) · 2020Review
- Advancing Genetic Tools inGenes · 2020Article
- Identification and characterization of a moonlighting protein-enolase for surface display in Streptococcus thermophilus.Microbial cell factories · 2020Article
- Article
- CRISPR/Cas9-Assisted Seamless Genome Editing in Lactobacillus plantarum and Its Application inApplied and environmental microbiology · 2019Article
- Targeted and Repetitive Chromosomal Integration Enables High-Level Heterologous Gene Expression inApplied and environmental microbiology · 2019Article
- A rapid and versatile tool for genomic engineering in Lactococcus lactis.Microbial cell factories · 2019Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundLactic acid bacteria (LAB) are receiving more attention to act as cell factories for the production of high-value metabolites. However, the molecular tools for genetic modifying these strains are mainly vector-based double-crossover strategies, which are laborious and inefficient. To address this problem, several counterselectable markers have been developed, while few of them could be used in the wild-type host cells without pretreatment.
resultsThe pheS gene encoding phenylalanyl-tRNA synthetase alpha subunit was identified in Lactococcus lactis NZ9000 genome. When mutant pheS gene (pheS*) under the control of the Lc. lactis NZ9000 L-lactate dehydrogenase promoter (P
conclusionsAs reported in E. coli, pheS* as a counterselectable marker has been demonstrated to be functional in targeted gene(s) deletion in Lc. lactis as well as in L. casei. Moreover, the efficiency and timesaving counterselectable seamless mutagenesis system PheS*/pG
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.