Evidence map›Paper›PMID 28679030›Full record

ArticleAngewandte Chemie (International ed. in English)2017

Reversible Product Release and Recapture by a Fungal Polyketide Synthase Using a Carnitine Acyltransferase Domain.

Leibniz Hang, Man-Cheng Tang, Colin J B Harvey, Claire G Page, Jian Li, Yiu-Sun Hung, Nicholas Liu, Maureen E Hillenmeyer, Yi Tang

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Angewandte Chemie (International ed. in English), 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.

0numbers the graph read from it
0cells of the map it votes in
11citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

11 citing papers in PubMed.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Leibniz HangDepartment of Chemistry and Biochemistry, Department of Chemical and Biomolecular Engineering, University of California, Los Angeles, CA, 90095, USA.
Man-Cheng TangDepartment of Chemistry and Biochemistry, Department of Chemical and Biomolecular Engineering, University of California, Los Angeles, CA, 90095, USA.
Colin J B HarveyStanford Genome Technology Center, Stanford University, Palo, CA, 93404, USA.
Claire G PageDepartment of Chemistry and Biochemistry, Department of Chemical and Biomolecular Engineering, University of California, Los Angeles, CA, 90095, USA.
Jian LiStanford Genome Technology Center, Stanford University, Palo, CA, 93404, USA.
Yiu-Sun HungDepartment of Chemistry and Biochemistry, Department of Chemical and Biomolecular Engineering, University of California, Los Angeles, CA, 90095, USA.
Nicholas LiuDepartment of Chemistry and Biochemistry, Department of Chemical and Biomolecular Engineering, University of California, Los Angeles, CA, 90095, USA.
Maureen E HillenmeyerStanford Genome Technology Center, Stanford University, Palo, CA, 93404, USA.
Yi TangDepartment of Chemistry and Biochemistry, Department of Chemical and Biomolecular Engineering, University of California, Los Angeles, CA, 90095, USA.

Funding

UCLA NIGMS T32 Program AssessmentT32GM007185 · NIGMS · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI TORRES, JORGE · 1985 to 2020
$21.7M
Genomic and synthetic biology tools for expressing natural product gene clustersU01GM110706 · NIGMS · STANFORD UNIVERSITY · PI DAVIS, RONALD WAYNE, STEINMETZ, LARS M · 2014 to 2018
$10.9M
MIRA: Enzymology and Self-Resistance of Natural Product BiosynthesisR35GM118056 · NIGMS · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI Yi Tang · 2016 to 2026
$6.1M
Rediscovering Natural Chemical Diversity -1DP1GM106413DP1GM106413 · NIGMS · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI TANG, YI · 2012 to 2016
$3.9M
Biotechnology Training in Biomedical Sciences and EngineeringT32GM067555 · NIGMS · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI GU, ZHEN · 2005 to 2019
$2.8M
NIGMS NIH HHS DP1 GM106413NIGMS NIH HHS R35 GM118056NIGMS NIH HHS T32 GM007185NIGMS NIH HHS T32 GM067555NIGMS NIH HHS U01 GM110706
6 · The paper itself

Abstract

Fungal polyketides have significant biological activities, yet the biosynthesis by highly reducing polyketide synthases (HRPKSs) remains enigmatic. An uncharacterized group of HRPKSs was found to contain a C-terminal domain with significant homology to carnitine O-acyltransferase (cAT). Characterization of one such HRPKS (Tv6-931) from Trichoderma virens showed that the cAT domain is capable of esterifying the polyketide product with polyalcohol nucleophiles. This process is readily reversible, as confirmed through the holo ACP-dependent transesterification of the released product. The methyltransferase (MT) domain of Tv6-931 can perform two consecutive α-methylation steps on the last β-keto intermediate to yield an α,α-gem-dimethyl product, a new programing feature among HRPKSs. Recapturing of the released product by cAT domain is suggested to facilitate complete gem-dimethylation by the MT.

Indexed as

BiocatalysisBiological ProductsCarnitine AcyltransferasesCatalytic DomainMetabolomicsMolecular StructurePolyketide SynthasesTrichodermaBiological ProductsCarnitine AcyltransferasesPolyketide Synthasesacyltransferasebiosynthesisheterologous expressionmethyltransferasepolyketides

Identifiers

PMID28679030
PMCPMC5580239

What OpenQuestion holds

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Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.