ArticleCurrent protocols in molecular biology2017
Mitochondrial Ribosome (Mitoribosome) Profiling for Monitoring Mitochondrial Translation In Vivo.
Article in Current protocols in molecular biology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed.
- Membrane insertion of mitochondrial-encoded proteins regulates ribosome decoding speed.Nature structural & molecular biology · 2026Article
- Translational activators align mRNAs at the small mitoribosomal subunit for translation initiation.Nature structural & molecular biology · 2026Article
- Multiple human enhancer RNAs contain long translated open reading frames.Genes & development · 2025Article
- A molecular switch at the yeast mitoribosomal tunnel exit controls cytochrome b synthesis.Nucleic acids research · 2025Article
- A molecular switch at the yeast mitoribosomal tunnel exit controls cytochromebioRxiv : the preprint server for biology · 2025Article
- Expanding TheCellVision.org: a central repository for visualizing and mining high-content cell imaging projects.Genetics · 2024Article
- Article
- DPC29 promotes post-initiation mitochondrial translation in Saccharomyces cerevisiae.Nucleic acids research · 2023Article
- Rapid Cryopurification of the Yeast Mitochondrial Ribosome.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Human Mitoribosome Profiling: A Re-engineered Approach Tailored to Study Mitochondrial Translation.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Mito-FUNCAT-FACS reveals cellular heterogeneity in mitochondrial translation.RNA (New York, N.Y.) · 2022Article
- Monitoring mammalian mitochondrial translation with MitoRiboSeq.Nature protocols · 2021Article
- Structural insights into dimethylation of 12S rRNA by TFB1M: indispensable role in translation of mitochondrial genes and mitochondrial function.Nucleic acids research · 2019Article
- Spt6 Is Required for the Fidelity of Promoter Selection.Molecular cell · 2018Article
- Following Ribosome Footprints to Understand Translation at a Genome Wide Level.Computational and structural biotechnology journal · 2018Review
- A Plant Biologist's Toolbox to Study Translation.Frontiers in plant science · 2018Review
- Using mitoribosomal profiling to investigate human mitochondrial translation.Wellcome open research · 2017Article
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2 authors.
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Abstract
Translation in the mitochondria is regulated by mechanisms distinct from those acting in the cytosol and in bacteria, yet precise methods for investigating it have lagged behind. This unit describes an approach, mitochondrial ribosome (mitoribosome) profiling, to quantitatively monitor mitochondrial translation with high temporal and spatial resolution in Saccharomyces cerevisiae. Mitoribosomes are immunoprecipitated from whole-cell lysate and the protected mRNA fragments are isolated. These fragments are then converted to sequencing libraries or analyzed by northern blot hybridization to reveal the distribution of mitoribosomes across the mitochondrial transcriptome. As information about RNA abundance is required to resolve translational from RNA effects, we also present an RNA sequencing approach that can be performed in parallel. Accurately capturing the biologically relevant distribution of mitoribosome positions depends on several critical parameters that are discussed. Application of mitoribosome profiling can reveal mechanisms of mitochondrial translational control that were not previously possible to uncover. © 2017 by John Wiley & Sons, Inc.
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