Evidence map›Paper›PMID 28678417›Full record

ArticleCurrent protocols in cytometry2017

Stochastic Optical Reconstruction Microscopy (STORM).

Jianquan Xu, Hongqiang Ma, Yang Liu

Abstract read
In one paragraph

Article in Current protocols in cytometry, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 53 papers.

0numbers the graph read from it
0cells of the map it votes in
53citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

53 citing papers in PubMed.

  1. Article
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  11. CFTR dictates monocyte adhesion by facilitating integrin clustering but not activation.Proceedings of the National Academy of Sciences of the United States of America · 2025
    Article
  12. Review
  13. Article
  14. Review
  15. A perspective of lipid nanoparticles for RNA delivery.Exploration (Beijing, China) · 2024
    Article
  16. Article
  17. Tracing the Chromatin: From 3C to Live-Cell Imaging.Chemical & biomedical imaging · 2024
    Review
  18. Article
  19. Article
  20. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Jianquan XuBiomedical and Optical Imaging Laboratory, Departments of Medicine and Bioengineering, University of Pittsburgh, Pittsburgh, Pennsylvania.
Hongqiang MaBiomedical and Optical Imaging Laboratory, Departments of Medicine and Bioengineering, University of Pittsburgh, Pittsburgh, Pennsylvania.
Yang LiuBiomedical and Optical Imaging Laboratory, Departments of Medicine and Bioengineering, University of Pittsburgh, University of Pittsburgh Cancer Institute, Pittsburgh, Pennsylvania.

Funding

Novel Nanoscale Single-Cell Analysis of Exfoliative CytologyR01EB016657 · NIBIB · UNIVERSITY OF PITTSBURGH AT PITTSBURGH · PI LIU, YANG · 2013 to 2016
$1.3M
(PQC2)Nanoscale changes in 3D nuclear architecture during breast tumorigenesisR01CA185363 · NCI · UNIVERSITY OF PITTSBURGH AT PITTSBURGH · PI LIU, YANG · 2014 to 2017
$1.3M
NCI NIH HHS R01 CA185363NIBIB NIH HHS R01 EB016657
6 · The paper itself

Abstract

Super-resolution (SR) fluorescence microscopy, a class of optical microscopy techniques at a spatial resolution below the diffraction limit, has revolutionized the way we study biology, as recognized by the Nobel Prize in Chemistry in 2014. Stochastic optical reconstruction microscopy (STORM), a widely used SR technique, is based on the principle of single molecule localization. STORM routinely achieves a spatial resolution of 20 to 30 nm, a ten-fold improvement compared to conventional optical microscopy. Among all SR techniques, STORM offers a high spatial resolution with simple optical instrumentation and standard organic fluorescent dyes, but it is also prone to image artifacts and degraded image resolution due to improper sample preparation or imaging conditions. It requires careful optimization of all three aspects-sample preparation, image acquisition, and image reconstruction-to ensure a high-quality STORM image, which will be extensively discussed in this unit. © 2017 by John Wiley & Sons, Inc.

Indexed as

AnimalsFluorescent DyesHumansMicroscopyMolecular ImagingStaining and LabelingFluorescent Dyessingle molecule localization microscopy (SMLM)stochastic optical reconstruction microscopy (STORM)super-resolution fluorescence microscopy

Identifiers

PMID28678417
PMCPMC5663316

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.